Otx1 Antibody (1F2) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # H00005013-M01
![ELISA: Otx1 Antibody (1F2) [H00005013-M01]](https://resources.bio-techne.com/images/products/Otx1-Antibody-1F2-ELISA-H00005013-M01-img0009.jpg "ELISA: Otx1 Antibody (1F2) [H00005013-M01]")
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Knockdown Validated, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 1F2
Format
Azide and BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
OTX1 (NP_055377, 10 a.a. ~ 116 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. YGMNGLGLAGPAMDLLHPSVGYPATPRKQRRERTTFTRSQLDVLEALFAKTRYPDIFMREEVALKINLPESRVQVWFKNRRAKCRQQQQSGSGTKSRPAKKKSSPVR
Specificity
OTX1 (1F2)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for Otx1 Antibody (1F2) - Azide and BSA Free
![Western Blot: Otx1 Antibody (1F2) [H00005013-M01]](https://resources.bio-techne.com/images/products/Otx1-Antibody-1F2-Western-Blot-H00005013-M01-img0008.jpg "Western Blot: Otx1 Antibody (1F2) [H00005013-M01]")
Western Blot: Otx1 Antibody (1F2) [H00005013-M01]
Western Blot: Otx1 Antibody (1F2) [H00005013-M01] - Analysis of OTX1 expression in transfected 293T cell line by OTX1 monoclonal antibody (M01), clone 1F2.Lane 1: OTX1 transfected lysate(37.3 KDa).Lane 2: Non-transfected lysate.![Immunocytochemistry/ Immunofluorescence: Otx1 Antibody (1F2) [H00005013-M01]](https://resources.bio-techne.com/images/products/Otx1-Antibody-1F2-Immunocytochemistry-Immunofluorescence-H00005013-M01-img0005.jpg "Immunocytochemistry/ Immunofluorescence: Otx1 Antibody (1F2) [H00005013-M01]")
Immunocytochemistry/ Immunofluorescence: Otx1 Antibody (1F2) [H00005013-M01]
Immunocytochemistry/Immunofluorescence: Otx1 Antibody (1F2) [H00005013-M01] - Analysis of monoclonal antibody to OTX1 on HeLa cell . Antibody concentration 10 ug/ml.![Western Blot: Otx1 Antibody (1F2) [H00005013-M01]](https://resources.bio-techne.com/images/products/Otx1-Antibody-1F2-Western-Blot-H00005013-M01-img0006.jpg "Western Blot: Otx1 Antibody (1F2) [H00005013-M01]")
Western Blot: Otx1 Antibody (1F2) [H00005013-M01]
Western Blot: Otx1 Antibody (1F2) [H00005013-M01] - Analysis of OTX1 over-expressed 293 cell line, cotransfected with OTX1 Validated Chimera RNAi ( Cat # H00005013-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with OTX1 monoclonal antibody (M01), clone 1F2 (Cat # H00005013-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.Applications for Otx1 Antibody (1F2) - Azide and BSA Free
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactivity against recombinant protein for WB. It has been used for RNAi Validation and ELISA.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Otx1
Long Name
Orthodenticle Homolog 1
Alternate Names
FLJ38361, homeobox protein OTX1, MGC15736, orthodenticle (Drosophila) homolog 1, orthodenticle homeobox 1, Orthodenticle homolog 1, orthodenticle homolog 1 (Drosophila)
Entrez Gene IDs
5013 (Human)
Gene Symbol
OTX1
OMIM
600036 (Human)
UniProt
Additional Otx1 Products
Product Documents for Otx1 Antibody (1F2) - Azide and BSA Free
Product Specific Notices for Otx1 Antibody (1F2) - Azide and BSA Free
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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