Osteopontin/OPN Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-1883
![Western Blot: Osteopontin/OPN Antibody [NB100-1883]](https://resources.bio-techne.com/images/products/Osteopontin-OPN-Antibody-Western-Blot-NB100-1883-img0001.jpg "Western Blot: Osteopontin/OPN Antibody [NB100-1883]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Mouse, Rat
Predicted:
Canine (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Peptide ELISA, Immunocytochemistry/ Immunofluorescence
Cited:
Immunohistochemistry-Frozen, Immunocytochemistry/ Immunofluorescence, IF/IHC
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-DPKSKEEDKHLK corresponding to C-Terminus according to NP_001035147.1, NP_000573.1, NP_001035149.1.
Reactivity Notes
Use in Rat reported in scientific literature (PMID:35063020). Mouse reactivity reported in scientific literature (PMID:23884286).
Localization
Secreted
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Osteopontin/OPN Antibody
Western Blot: Osteopontin/OPN Antibody [NB100-1883]
Western Blot: Osteopontin/OPN Antibody [NB100-1883] - Staining of lysates of cell line MOLT4 (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Applications for Osteopontin/OPN Antibody
Application
Recommended Usage
Peptide ELISA
Detection limit 1:32000
Western Blot
0.1-0.3 ug/ml
Application Notes
Use in Immunohistochemistry-Frozen reported in scientific literature (PMID:35063020) WB: Approx 60kDa band observed in lysates of Human Tonsil and lysates of cell line MOLT4 and Jurkat (calculated MW of 33.8kDa according to NP_000573.1). The observed molecular weight corresponds to earlier findings in literature (Singh et al, J Biol Chem. 1995 Nov 24;270(47):28471-8; PMID: 7499354). Primary incubation was 1 hour. ICC/IF, IHC usage is reported in scientific literature (PMID: 23884286).
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Osteopontin/OPN
Long Name
Secreted Phosphoprotein 1 [BNSP]
Alternate Names
Eta-1, OPN, Spp1
Gene Symbol
SPP1
UniProt
Additional Osteopontin/OPN Products
Product Documents for Osteopontin/OPN Antibody
Product Specific Notices for Osteopontin/OPN Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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