Osteopontin/OPN Antibody (OSP/8693R)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-20504
Recombinant Monoclonal Antibody
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat, Canine, Mink, Rabbit, Turkey
Applications
Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG1 kappa Clone # OSP/8693R
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Recombinant full-length rat Osteopontin/OPN protein
Localization
Secreted.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG1 kappa
Description
Positive Controls: Bone. Found in plasma.
Antibody with azide - store at 2 to 8C. Non-hazardous. No MSDS required.
Antibody with azide - store at 2 to 8C. Non-hazardous. No MSDS required.
Scientific Data Images for Osteopontin/OPN Antibody (OSP/8693R)
Osteopontin/OPN Antibody (OSP/8693R)
Formalin-fixed, paraffin-embedded human brain stained with Osteopontin/OPN antibody (OSP/8693R). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.Applications for Osteopontin/OPN Antibody (OSP/8693R)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Western Blot
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: Osteopontin/OPN
Long Name
Secreted Phosphoprotein 1 [BNSP]
Alternate Names
Eta-1, OPN, Spp1
Gene Symbol
SPP1
UniProt
Additional Osteopontin/OPN Products
Product Documents for Osteopontin/OPN Antibody (OSP/8693R)
Product Specific Notices for Osteopontin/OPN Antibody (OSP/8693R)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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