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Osteopontin/OPN Antibody (OSP/4589) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-49523

Novus Biologicals, part of Bio-Techne
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NBP3-49523

Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine, Mink, Rabbit, Turkey

Applications

Immunohistochemistry, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Functional

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG1 Clone # OSP/4589

Format

Azide and BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant full-length rat Spp1 protein (Uniprot: P10451)

Localization

Secreted

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Theoretical MW

34.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Prepared in 10mM PBS WITHOUT BSA & azide at 1.0mg/ml. Also availabe with 0.05% BSA & 0.05% azide.

Antibody with azide - store at 2 to 8 C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non-hazardous.

Scientific Data Images for Osteopontin/OPN Antibody (OSP/4589) - Azide and BSA Free

Osteopontin/OPN Antibody (OSP/4589) - Azide and BSA Free

Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody (OSP/4589) - Azide and BSA Free [NBP3-49523] -

Formalin-fixed, paraffin-embedded human brain stained with Osteopontin/OPN Antibody (OSP/4589) - Azide and BSA Free. Inset: PBS instead of primary antibody; secondary only negative control.
Osteopontin/OPN Antibody (OSP/4589) - Azide and BSA Free

Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody (OSP/4589) - Azide and BSA Free [NBP3-49523] -

IHC analysis of formalin-fixed, paraffin-embedded human prostate stained by Osteopontin/OPN Antibody (OSP/4589) - Azide and BSA Free at 2ug/ml in PBS. HIER: Tris/EDTA, pH9.0, 45min. 2°: HRP-polymer, 30min. DAB, 5min.

Applications for Osteopontin/OPN Antibody (OSP/4589) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Functional

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Osteopontin/OPN

Osteopontin is the principal phosphorylated glycoprotein of bone and is expressed in a limited number of other tissues including dentine. Osteopontin is produced by osteoblasts under stimulation by calcitriol and binds tightly to hydroxyapatite. It is also involved in the anchoring of osteoclasts to the mineral of bone matrix via the vitronectin receptor, which has specificity for osteopontin. Osteopontin is overexpressed in a variety of cancers, including lung, breast, colorectal, stomach, ovarian, melanoma and mesothelioma.

Long Name

Secreted Phosphoprotein 1 [BNSP]

Alternate Names

Eta-1, OPN, Spp1

Gene Symbol

SPP1

Additional Osteopontin/OPN Products

Product Documents for Osteopontin/OPN Antibody (OSP/4589) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Osteopontin/OPN Antibody (OSP/4589) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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