Osteopontin/OPN Antibody (OSP/4589)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-48989
Key Product Details
Species Reactivity
Human, Mouse, Rat, Canine, Mink, Rabbit, Turkey
Applications
Immunohistochemistry, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Functional
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG1 Clone # OSP/4589
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant full-length rat Spp1 protein (Uniprot: P10451)
Localization
Secreted
Clonality
Monoclonal
Host
Rat
Isotype
IgG1
Theoretical MW
34.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of Ab produced in HEK293 cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody with azide - store at 2 to 8 C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Antibody with azide - store at 2 to 8 C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Scientific Data Images for Osteopontin/OPN Antibody (OSP/4589)
Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody (OSP/4589) [NBP3-48989] -
Formalin-fixed, paraffin-embedded human brain stained with Osteopontin/OPN Antibody (OSP/4589). Inset: PBS instead of primary antibody; secondary only negative control.
Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody (OSP/4589) [NBP3-48989] -
IHC analysis of formalin-fixed, paraffin-embedded human prostate stained by Osteopontin/OPN Antibody (OSP/4589) at 2ug/ml in PBS. HIER: Tris/EDTA, pH9.0, 45min. 2°: HRP-polymer, 30min. DAB, 5min.Applications for Osteopontin/OPN Antibody (OSP/4589)
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Functional
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Osteopontin/OPN
Long Name
Secreted Phosphoprotein 1 [BNSP]
Alternate Names
Eta-1, OPN, Spp1
Gene Symbol
SPP1
Additional Osteopontin/OPN Products
Product Documents for Osteopontin/OPN Antibody (OSP/4589)
Product Specific Notices for Osteopontin/OPN Antibody (OSP/4589)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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