Osteopontin/OPN Antibody (CL10686) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07980

Catalog #
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NBP3-07980-100ul
NBP3-07980-25ul
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Conjugate
Catalog #
Alexa Fluor 350
NBP3-44052AF350
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Alexa Fluor 405
NBP3-44052AF405
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Alexa Fluor 488
NBP3-44052AF488
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Alexa Fluor 532
NBP3-44052AF532
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Alexa Fluor 594
NBP3-44052AF594
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Alexa Fluor 647
NBP3-44052AF647
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Alexa Fluor 700
NBP3-44052AF700
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Alexa Fluor 750
NBP3-44052AF750
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Alexa Fluor Plus 405
NBP3-44052AFP405
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Alexa Fluor Plus 488
NBP3-44052AFP488
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Alexa Fluor Plus 555
NBP3-44052AFP555
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Alexa Fluor Plus 594
NBP3-44052AFP594
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Alexa Fluor Plus 647
NBP3-44052AFP647
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Alexa Fluor Plus 680
NBP3-44052AFP680
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Allophycocyanin
NBP3-44052APC
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Biotin
NBP3-44052B
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CoraFluor 1
NBP3-44052CL1
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DyLight 350
NBP3-44052UV
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DyLight 405
NBP3-44052V
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DyLight 488
NBP3-44052G
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DyLight 550
NBP3-44052R
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DyLight 594
NBP3-44052DL594
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DyLight 650
NBP3-44052C
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DyLight 680
NBP3-44052FR
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DyLight 755
NBP3-44052IR
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FITC
NBP3-44052F
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HRP
NBP3-44052H
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Janelia Fluor 525
NBP3-44052JF525
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Janelia Fluor 549
NBP3-44052JF549
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Janelia Fluor 646
NBP3-44052JF646
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Janelia Fluor 669
NBP3-44052JF669
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mFluor Violet 450 SE
NBP3-44052MFV450
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mFluor Violet 500 SE
NBP3-44052MFV500
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mFluor Violet 610 SE
NBP3-44052MFV610
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PE
NBP3-44052PE
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PerCP
NBP3-44052PCP
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL10686

Format

BSA Free

Concentration

1 mg/ml

View all Osteopontin/OPN Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from P10451, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Osteopontin/OPN Antibody (CL10686) - BSA Free

Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody (CL10686) [NBP3-07980]

Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody (CL10686) [NBP3-07980]

Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody (CL10686) [NBP3-07980] - Analysis in human cerebral cortex and tonsil tissues using NBP3-07980 antibody. Corresponding Osteopontin/OPN RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody (CL10686) [NBP3-07980]

Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody (CL10686) [NBP3-07980]

Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody (CL10686) [NBP3-07980] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody (CL10686) [NBP3-07980]

Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody (CL10686) [NBP3-07980]

Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody (CL10686) [NBP3-07980] - Staining of human cerebellum shows moderate cytoplasmic positivity in microglia.
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Applications for Osteopontin/OPN Antibody (CL10686) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Osteopontin/OPN

Osteopontin is the principal phosphorylated glycoprotein of bone and is expressed in a limited number of other tissues including dentine. Osteopontin is produced by osteoblasts under stimulation by calcitriol and binds tightly to hydroxyapatite. It is also involved in the anchoring of osteoclasts to the mineral of bone matrix via the vitronectin receptor, which has specificity for osteopontin. Osteopontin is overexpressed in a variety of cancers, including lung, breast, colorectal, stomach, ovarian, melanoma and mesothelioma.

Long Name

Secreted Phosphoprotein 1 [BNSP]

Alternate Names

Eta-1, OPN, Spp1

Gene Symbol

SPP1

Additional Osteopontin/OPN Products

Product Documents for Osteopontin/OPN Antibody (CL10686) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Osteopontin/OPN Antibody (CL10686) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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