non-muscle Myosin IIA [p Ser1943] Antibody (GT144) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13579
Key Product Details
Validated by
Knockout/Knockdown, Biological Validation
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # GT144
Format
Azide and BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Carrier-protein conjugated synthetic peptide surrounding phospho Ser1943 of human non-muscle Myosin IIA. The exact sequence is proprietary.
Modification
p Ser1943
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
227 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Centrifuge briefly prior to opening.
Scientific Data Images
Western Blot: non-muscle Myosin IIA [p Ser1943] Antibody (GT144) [NBP3-13579] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with non-muscle Myosin IIA (phospho Ser1943) antibody [GT144] (NBP3-13579) diluted at 1:10000. The HRP-conjugated anti-mouset IgG antibody (NBP2-19382) was used to detect the primary antibody.
Immunohistochemistry-Paraffin: non-muscle Myosin IIA [p Ser1943] Antibody (GT144) [NBP3-13579] - Human mammary gland. non-muscle Myosin IIA (phospho Ser1943) antibody [144] (NBP3-13579) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Western Blot: non-muscle Myosin IIA [p Ser1943] Antibody (GT144) [NBP3-13579] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with non-muscle Myosin IIA(phospho Ser1943) antibody [GT144] (NBP3-13579) diluted at 1:20000.
![non-muscle Myosin IIA [p Ser1943] Antibody (GT144) - Azide and BSA Free](https://resources.bio-techne.com/images/products/nbp3-13579_mouse-non-muscle-myosin-iia-p-ser-1943-mab-gt144-immunocytochemistry-immunofluorescence-2611202518213311.jpg "Immunocytochemistry/ Immunofluorescence: non-muscle Myosin IIA [p Ser1943] Antibody (GT144) [NBP3-13579] -")
Applications
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
1:5000-1:20000
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Non-muscle Myosin IIA
Long Name
Myosin-9
Alternate Names
MYH9, NMMHC II-a, NMMHC-A, NMMHC-IIA
Gene Symbol
MYH9
Additional Non-muscle Myosin IIA Products
Product Specific Notices
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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