NKX6.1 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-49672

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Fish - Danio rerio (Zebrafish)

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, In vitro assay, Simple Western, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, In vitro assay, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all NKX6.1 Antibodies »

Product Specifications

Immunogen

A genomic peptide made to an internal region of the human Nkx6.1 protein (within residues 50-200). [Swiss-Prot P78426]

Reactivity Notes

Rat reactivity reported from a verified customer review.

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NKX6.1 Antibody - BSA Free

Western Blot: NKX6.1 AntibodyBSA Free [NBP1-49672]

Western Blot: NKX6.1 AntibodyBSA Free [NBP1-49672]

Western Blot: NKX6.1 Antibody [NBP1-49672] - Analysis of Nkx6.1 in human skeletal muscle.
Immunocytochemistry/ Immunofluorescence: NKX6.1 Antibody - BSA Free [NBP1-49672]

Immunocytochemistry/ Immunofluorescence: NKX6.1 Antibody - BSA Free [NBP1-49672]

Immunocytochemistry/Immunofluorescence: NKX6.1 Antibody [NBP1-49672] - Nkx6.1 antibody was tested at 1:250 in INS-1 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red). Image objective 40X.
Immunohistochemistry: NKX6.1 Antibody - BSA Free [NBP1-49672]

Immunohistochemistry: NKX6.1 Antibody - BSA Free [NBP1-49672]

NKX6.1-Antibody---BSA-Free-Immunohistochemistry-NBP1-49672-img0012.jpg
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Applications for NKX6.1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:100

Immunohistochemistry-Paraffin

1:50 - 1:100

In vitro assay

reported in scientific literature (PMID 30471425)

Simple Western

1:100

Western Blot

1:100 - 1:2000
Application Notes
Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in BTC-6 lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:100. Separated by Size-Wes, Sally Sue/Peggy Sue.

Reviewed Applications

Read 4 reviews rated 4.8 using NBP1-49672 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS, 30% Glycerol

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NKX6.1

NKX6.1 (homeobox protein Nkx-6.1) is a transcription factor that binds to specific A/T-rich sequences in promoter regions of several genes and is known for its involvement in transcriptional regulation in islet beta cells via NKX6.1-insulin promoter binding mediated control of insulin gene. Together with NKX2-2 as well as IRX3, NKX6.1 acts to restrict the generation of motor neurons to the appropriate region of the neural tube. It belongs to neuronal progenitor factors class II proteins that are induced by SHH signals and is a member of the homeodomain family of transcription factors responsible for multiple organ systems development. The pancreatic development involves many such homeodomain proteins including Hb9, Pdx1, Pax4, Nkx2.2, and importantly on Nkx6.1 and mice deficient for Nkx6.1 display a dramatic reduction in beta-cell numbers in pancreas with near-normal development of other glandular cell types. Moreover, Nkx6.1 deficient mice have significant defects involving V2 interneuron and motor neuron specification in the ventral spinal cord, thereby emphasizing an important parallel in developmental pathways leading to beta-cells and neurons.

Long Name

NK6 Homeobox 1

Alternate Names

NKX6-1, NKX6A

Entrez Gene IDs

4825 (Human); 18096 (Mouse)

Gene Symbol

NKX6-1

UniProt

P78426 (Human)

Additional NKX6.1 Products

Product Documents for NKX6.1 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for NKX6.1 Antibody - BSA Free

Manufactured by Genomic Antibody Technology™. GAT FAQs

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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