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Nicotinic Acetylcholine R alpha 5/CHRNA5 Antibody (2D2D10) - BSA Free

Catalog # NBP2-61677 | Novus Biologicals a Bio-Techne Brand
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NBP2-61677
NBP2-61677-0.025ml

Key Product Details

Species Reactivity

Human, Rat

Applications

ELISA, Flow Cytometry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 2D2D10

Format

BSA Free

Concentration

1 mg/ml

Product Summary for Nicotinic Acetylcholine R alpha 5/CHRNA5 Antibody (2D2D10) - BSA Free

Immunogen

Purified recombinant fragment of human CHRNA5 (AA: extra 23-254) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Nicotinic Acetylcholine R alpha 5/CHRNA5 Antibody (2D2D10) - BSA Free

Western Blot: Nicotinic Acetylcholine R alpha 5/CHRNA5 Antibody (2D2D10) [NBP2-61677] - Analysis using CHRNA5 mAb against HEK293 (1) and CHRNA5 (AA: 23-254)-hIgGFc transfected HEK293 (2) cell lysate.
Flow Cytometry: Nicotinic Acetylcholine R alpha 5/CHRNA5 Antibody (2D2D10) [NBP2-61677] - Analysis of SK-N-SH cells using CHRNA5 mouse mAb (green) and negative control (red).
Western Blot: Nicotinic Acetylcholine R alpha 5/CHRNA5 Antibody (2D2D10) [NBP2-61677] - Analysis using CHRNA5 mAb against human CHRNA5 (AA: 23-254) recombinant protein. (Expected MW is 52.5 kDa)

Applications for Nicotinic Acetylcholine R alpha 5/CHRNA5 Antibody (2D2D10) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Flow Cytometry

1:10-1:1000

Western Blot

1:100-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Nicotinic Acetylcholine R alpha 5/CHRNA5

The protein encoded by this gene is a nicotinic acetylcholine receptor subunit and a member of a superfamily of ligand-gated ion channels that mediate fast signal transmission at synapses. These receptors are thought to be heteropentamers composed of separate but similar subunits. Defects in this gene have been linked to susceptibility to lung cancer type 2 (LNCR2).

Long Name

Nicotinic Acetylcholine Receptor alpha 5

Alternate Names

CHRNA5, NACHRA5, Nicotinic Acetylcholine Ra5

Gene Symbol

CHRNA5

Product Documents for Nicotinic Acetylcholine R alpha 5/CHRNA5 Antibody (2D2D10) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Nicotinic Acetylcholine R alpha 5/CHRNA5 Antibody (2D2D10) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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