NFATC2/NFAT1 Antibody (24GB360)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-50525

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NBP3-50525-100ul
NBP3-50525-20ul
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Key Product Details

Species Reactivity

Human, Rat

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 24GB360

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all NFATC2 Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human NFATC2/NFAT1 (Uniprot: Q13469)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NFATC2/NFAT1 Antibody (24GB360)

NFATC2/NFAT1 Antibody (24GB360)

Immunocytochemistry/ Immunofluorescence: NFATC2/NFAT1 Antibody (24GB360)[NBP3-50525] -

Immunocytochemical staining of HAP-1 cells with NFATC2/NFAT1 antibody (1:1,000). Nuclei were stained blue with DAPI; NFATC2/NFAT1 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.
NFATC2/NFAT1 Antibody (24GB360)

Flow Cytometry: NFATC2/NFAT1 Antibody (24GB360)[NBP3-50525] -

Flow cytometric analysis of NFATC2/NFAT1expression in HAP-1 cells using NFATC2/NFAT1 antibody (1:2,000). Green, isotype control; red, NFATC2.
NFATC2/NFAT1 Antibody (24GB360)

Western Blot: NFATC2/NFAT1 Antibody (24GB360)[NBP3-50525] -

Western blotting analysis using NFATC2/NFAT1 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.

Applications for NFATC2/NFAT1 Antibody (24GB360)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NFATC2

The nuclear factor of activated T-cells (NFAT) transcription complex is required for the expression of a group of proteins that collectively regulate the immune response. Four NFAT proteins, encoded on separate genes and expressed as several splice variants, have been described: NFAT1, NFAT2, NFAT3, and NFAT4. These proteins show a low level of sequence similarity with the Dorsal/Rel/NFkB family of transcription factors. Another NFAT-related protein termed NFAT5 differs from isoforms 1-4 in that it lacks many of the Fos/Jun contact sites observed in its predecessors and its subcellular localization is not calcineurin-dependent.

Long Name

Nuclear factor of activated T-cells, cytoplasmic 2

Alternate Names

NFAT1, NFATP

Gene Symbol

NFATC2

Additional NFATC2 Products

Product Documents for NFATC2/NFAT1 Antibody (24GB360)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for NFATC2/NFAT1 Antibody (24GB360)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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