Neuromedin-U Antibody (HL3549)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-48826
Recombinant Monoclonal Antibody
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Human
Applications
Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL3549
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fragment of human Neuromedin-U.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit Neuromedin-U Antibody (HL3549) (NBP3-48826) is a recombinant monoclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Neuromedin-U Antibody (HL3549)
Immunocytochemistry/ Immunofluorescence: Neuromedin-U Antibody (HL3549) [NBP3-48826] -
Neuromedin-U antibody [HL3549] detects Neuromedin-U protein by immunofluorescent analysis.Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Neuromedin-U stained by Neuromedin-U antibody [HL3549] (NBP3-48826) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Fluoroshield with DAPI .
Western Blot: Neuromedin-U Antibody (HL3549) [NBP3-48826] -
K562 whole cell extract and conditioned medium (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Neuromedin-U antibody [HL3549] (NBP3-48826) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Western Blot: Neuromedin-U Antibody (HL3549) [NBP3-48826] -
Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Neuromedin-U antibody [HL3549] (NBP3-48826) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.Applications for Neuromedin-U Antibody (HL3549)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Neuromedin-U
Alternate Names
NMU
Gene Symbol
NMU
Additional Neuromedin-U Products
Product Documents for Neuromedin-U Antibody (HL3549)
Product Specific Notices for Neuromedin-U Antibody (HL3549)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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