NEK2 Antibody (23GB2085)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-50538
Key Product Details
Species Reactivity
Human
Applications
Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated
Label
Unconjugated
Antibody Source
Monoclonal Rabbit IgG Clone # 23GB2085
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthesized peptide derived from human NEK2 (Uniprot: P51955)
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for NEK2 Antibody (23GB2085)
Immunocytochemistry/ Immunofluorescence: NEK2 Antibody (23GB2085)[NBP3-50538] -
Immunocytochemical staining of HepG2 cells with NEK2 antibody (1:1,000). Nuclei were stained blue with DAPI; NEK2 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 um.
Flow Cytometry: NEK2 Antibody (23GB2085)[NBP3-50538] -
Flow cytometric analysis of NEK2expression in HepG2 cells using NEK2 antibody (1:2,000). Green, isotype control; red, NEK2.
Western Blot: NEK2 Antibody (23GB2085)[NBP3-50538] -
Western blotting analysis using NEK2 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.Applications for NEK2 Antibody (23GB2085)
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Knockdown Validated
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.4), 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: NEK2
Long Name
NIMA [Never In Mitosis Gene A]-related Kinase 2
Alternate Names
HsPK21, NLK1
Gene Symbol
NEK2
Additional NEK2 Products
Product Documents for NEK2 Antibody (23GB2085)
Product Specific Notices for NEK2 Antibody (23GB2085)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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