Myosin 5a Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-92156
![Myosin 5a Antibody - BSA Free Western Blot: Myosin 5a Antibody - BSA Free [NBP1-92156]](https://resources.bio-techne.com/images/products/nbp1-92156_rabbit-polyclonal-myosin-5a-antibody-295202516234113.jpg "Western Blot: Myosin 5a Antibody - BSA Free [NBP1-92156]")
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Predicted:
Rat (99%). Backed by our 100% Guarantee.
Applications
Validated:
Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated
Cited:
Immunocytochemistry/ Immunofluorescence, IF/IHC, Knockdown Validated
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: QNLQLPPEARIEASLQHEITRLTNENLDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENISPGQIIDEPIRPVNIPRKEKDFQGMLEYKKEDEQKLVKNLILELKPRGVAVNLIPG
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID:32815283)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Myosin 5a Antibody - BSA Free
![Immunocytochemistry/ Immunofluorescence: Myosin 5a Antibody [NBP1-92156]](https://resources.bio-techne.com/images/products/Myosin-5a-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-92156-img0005.jpg "Immunocytochemistry/ Immunofluorescence: Myosin 5a Antibody [NBP1-92156]")
Immunocytochemistry/ Immunofluorescence: Myosin 5a Antibody [NBP1-92156]
Immunocytochemistry/Immunofluorescence: Myosin 5a Antibody [NBP1-92156] - Staining of human cell line A-431 shows localization to microtubule organizing center & focal adhesion sites. Antibody staining shown in green.![Myosin 5a Antibody - BSA Free Immunohistochemistry: Myosin 5a Antibody - BSA Free [NBP1-92156]](https://resources.bio-techne.com/images/products/nbp1-92156_rabbit-polyclonal-myosin-5a-antibody-30520251010572.jpg "Immunohistochemistry: Myosin 5a Antibody - BSA Free [NBP1-92156]")
Immunohistochemistry: Myosin 5a Antibody - BSA Free [NBP1-92156]
Staining of human liver shows no positivity in hepatocytes as expected.Applications for Myosin 5a Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Knockdown Validated
Validation (Barthelemy-Requin et al).
Western Blot
0.04-0.4 µg/ml
Application Notes
IHC-P, Retrieval method HIER pH6, ICC/IF, Fixation/Permeabilization: PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Myosin 5a
Alternate Names
Dilute myosin heavy chain, non-muscle, GS1myosin, heavy polypeptide kinase, MYH12myosin-12, MYO5, Myosin heavy chain 12, myosin V, myosin VA (heavy chain 12, myoxin), myosin VA (heavy polypeptide 12, myoxin), Myosin-12, myosin-Va, myoxin, MYR12
Gene Symbol
MYO5A
Additional Myosin 5a Products
Product Documents for Myosin 5a Antibody - BSA Free
Product Specific Notices for Myosin 5a Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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