Myeloperoxidase/MPO Antibody (2C7) [mFluor Violet 450 SE]
Novus Biologicals, part of Bio-Techne | Catalog # NB100-64803MFV450
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, ELISA, Flow Cytometry
Label
mFluor Violet 450 SE (Excitation = 406 nm, Emission = 445 nm)
Antibody Source
Monoclonal Mouse IgG1 Clone # 2C7
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Human myeloperoxidase
Reactivity Notes
Predicted cross-reactivities: Dog
Specificity
NB100-64803 recognizes human myeloperoxidase (MPO). MPO is an important component of azurophilic granules in neutrophils, being involved in microbicidal processes. The protein is a multimer of 2 heavy chains (55kD) and two light chains (15kD), the heavy chains being linked by a disulphide bond. NB100-64803 recognizes native MPO in Western blots, and the heavy chain following boiling of the sample. The antibody also recognizes recombinant MPO in Western blots and weakly in ELISA. The antibody may be of value in the study of myeloid cells and myeloid leukaemias by flow cytometry following cell permeablisation.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Description
This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
Scientific Data Images for Myeloperoxidase/MPO Antibody (2C7) [mFluor Violet 450 SE]
Myeloperoxidase/MPO Antibody (2C7) [mFluor Violet 450 SE] [NB100-64803MFV450] -
Myeloperoxidase/MPO Antibody (2C7) [mFluor Violet 450 SE] [NB100-64803MFV450] - Vial of mFluor Violet 450 conjugated antibody. mFluor Violet 450 is optimally excited at 406 nm by the Violet laser (405 nm) and has an emission maximum of 445 nm.Applications for Myeloperoxidase/MPO Antibody (2C7) [mFluor Violet 450 SE]
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Frozen
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Recommended applications are based on validated applications from the unconjugated base product NB100-64803. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectra ViewerFormulation, Preparation, and Storage
Purification
Protein G purified
Formulation
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: Myeloperoxidase/MPO
Alternate Names
MPO
Gene Symbol
MPO
Additional Myeloperoxidase/MPO Products
Product Documents for Myeloperoxidase/MPO Antibody (2C7) [mFluor Violet 450 SE]
Product Specific Notices for Myeloperoxidase/MPO Antibody (2C7) [mFluor Violet 450 SE]
mFluor(TM) is a trademark of AAT Bioquest, Inc. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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