Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody (31-1D1)
Novus Biologicals, part of Bio-Techne | Catalog # NB120-2805
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Porcine
Cited:
Rat
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 31-1D1
Concentration
2 mg/ml
Product Specifications
Immunogen
Purified porcine cardiac m2 muscarinic acetylcholine receptor.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Localization
Plasma membrane
Specificity
This is specific for the m2 mAChR subtype.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Description
Novus Biologicals Mouse Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody (31-1D1) (NB120-2805) is a monoclonal antibody validated for use in IHC, WB, Flow, ICC/IF and IP. Anti-Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody (31-1D1)
Immunohistochemistry-Paraffin: Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody (31-1D1) [NB120-2805]
Immunohistochemistry-Paraffin: Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody (31-1D1) [NB120-2805] - Human kidney tissue.Western Blot: Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody (31-1D1) [NB120-2805] -
Purinergic and muscarinic signaling associated molecules changes on western blot study in separated group. Figure 7 showed the expression of muscarinic cholinergic (M2 and M3) and purinergic receptor proteins (P2X2 and P2X3) by Western blot. In the top panel, (a) showed the expression of M2 receptor is significantly decreased in BPAO + ADSCs group compared to BAPO group (# p < 0.05). The expression of M2 receptor had trend of decreasing in BPAO + ADSC-derived MVs group compared to BAPO group without significant differences (p > 0.05). In the middle panel, (b) showed that the expression of M3 receptor was significantly increased in BPAO group compared to sham group (* p < 0.05). The expression of M3 receptor is significantly decreased in BPAO + ADSC-derived MVs group compared to BAPO group (# p < 0.05). (c) showed the expression of P2X2 receptors was significantly increased in BPAO group compared to sham group (* p < 0.05). The expression of P2X2 receptor is significantly decreased in BPAO + ADSC-derived MVs and BAPO + MVs group compared to BAPO group (#p < 0.05). In the bottom panel, (d) showed the expression of P2X3 receptor is of no significant differences between groups. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34210091), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody (31-1D1)
Application
Recommended Usage
Flow Cytometry
1:10 - 1:1000
Immunocytochemistry/ Immunofluorescence
2 ug/ml
Immunohistochemistry
1:10 - 1:500
Immunohistochemistry-Paraffin
1:20
Immunoprecipitation
1:10 - 1:500
Western Blot
1:1000
Application Notes
WB: Detects 60 - 80 kDa protein(s) (depending on the degree of glycosylation) representing the m2AChR / CHRM2.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS with 1 mg/ml BSA
Preservative
0.05% Sodium Azide
Concentration
2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: CHRM2
Long Name
Cholinergic Receptor Muscarinic 2
Alternate Names
HM2, mAChR M2
Gene Symbol
CHRM2
Additional CHRM2 Products
Product Documents for Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody (31-1D1)
Product Specific Notices for Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody (31-1D1)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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