Mouse Wnt-5a Biotinylated Antibody

Name: Mouse Wnt-5a Biotinylated Antibody
Brand: R&D Systems
SKU: BAF645
Price: 424 EUR

R&D Systems, Inc. a Bio-Techne Brand

Catalog # BAF645

Citations (4)

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Species Reactivity:	Mouse
Immunogen:	E. coli-derived recombinant mouse Wnt‑5a peptide Gln254-Cys334 Accession # P22725
Applications:	Western Blot
Label:	Biotin
Source:	Polyclonal Goat IgG
Specificity:	Detects mouse Wnt-5a in Western blots. In this format, approximately 2% cross-reactivity with recombinant mouse Wnt‑4 is observed.

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Applications for Mouse Wnt-5a Biotinylated Antibody

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Mouse Wnt‑5a (Catalog # 645-WN)

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: Wnt-5a

Wnt proteins are secreted glycoproteins that contain a conserved pattern of 23 - 24 cysteine residues. Wnts play critical roles in both carcinogenesis and embryonic development for a variety of organisms. Wnts bind to receptors of the Frizzled family, sometimes in conjunction with other membrane-associated proteins such as LRPs or proteoglycans. Downstream effects of Wnt signaling occur through different intracellular components, depending on which pathway is activated. Three pathways have been characterized: the canonical Wnt/ beta -catenin pathway, the Wnt/Ca²⁺ pathway, and the planar cell polarity (1 - 2).

Wnt-5a is part of the subgroup of Wnts that are not axis-inducing in Xenopus embryos and do not transform C57MG mammary epithelial cells. This subgroup is also implicated in the Wnt/Ca2+ pathway, playing roles in cell movements and cell adhesion (3). This non-canonical Wnt pathway can inhibit canonical Wnt/ beta -catenin signaling. In Wnt-5a deficient mouse embryos, beta -catenin accumulates in the limb bud suggesting that Wnt-5a normally promotes degradation of beta -catenin (4). Likewise, in Xenopus embryos Wnt-5a antagonizes the ability of the canonical Wnt subgroup to induce a secondary axis (5). Wnt-5a is implicated in various types of cancer and has complex roles. It acts as a tumor suppressor for mammary, B-cell, colon, and uroepithelial cancer cells but is up-regulated in melanomas, where expression levels correlate with severity of metastasis (3). Furthermore, aberrant Wnt-5a signaling results in other diseases such as rheumatoid arthritis (6). Like other developmental growth factors Wnt-5a has diverse roles in development. They are too numerous to enunciate here, as functions span from early anterior-posterior development and gastrulation movements to maintaining hematopoietic stem cell population, lung morphogenesis, and limb outgrowth. Mouse and human Wnt-5a share 97% amino acid identity.

Item	Value
References	Miller, J.R. (2002) Genome Biol. 3:3001. Roelink, H. and R. Nusse (1991) Genes Dev. 5:381. Veeman, M.T. et al. (2003) Developmental Cell 5:367. Topol, L. et al. (2003) J. Cell Biol 162:899. Torres, M. et al. (1996) J. Cell Biol. 133:1123. Sen, M. et al. (2001) Arthritis & Rheumatism 44:772.
Long Name	Wingless-type MMTV Integration Site Family, Member 5a
Entrez Gene IDs	7474 (Human); 22418 (Mouse)
Alternate Names	WNT-5A protein, Wnt-5a, Wnt5a, hWNT5A, protein Wnt-5a, wingless-type MMTV integration site family, member 5A

Citations for Mouse Wnt-5a Biotinylated Antibody (4)

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Citations are publications that use Bio-Techne products. Selected citations for Mouse Wnt-5a Biotinylated Antibody include:

A Ndoye et al. (2017), ATG5 mediates a positive feedback loop between Wnt signaling and autophagy in melanoma Cancer Res., 0(0).
PMID: 28887323
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

R Behera et al. (2017), Inhibition of age-related therapy resistance in melanoma by rosiglitazone-mediated induction of Klotho Clin. Cancer Res, 0(0).
PMID: 28232477
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

O'Connell MP et al. (2010), The orphan tyrosine kinase receptor, ROR2, mediates Wnt5A signaling in metastatic melanoma. Oncogene, 29(1):34-44.
PMID: 19802008
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

O'Connell MP et al. (2009), Wnt5A activates the calpain-mediated cleavage of filamin A. J. Invest. Dermatol., 129(7):1782-9.
PMID: 19177143

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Mouse Wnt-5a Biotinylated Antibody

R&D Systems, Inc. a Bio-Techne Brand

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