Mouse/Rat Wnt-5a Antibody
R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Species Reactivity: | Mouse, Rat |
Immunogen: |
E. coli-derived recombinant mouse Wnt‑5a peptide Gln254-Cys334 Accession # P22725 |
Applications: | Immunohistochemistry, Western Blot |
Label: | Unconjugated |
Source: | Polyclonal Goat IgG |
Specificity: |
Detects mouse and rat Wnt-5a in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant mouse (rm) Wnt‑5b is observed and less than 2% cross-reactivity with rmWnt-1, rmWnt-3a, rmWnt-4, rmWnt-11, and rmWnt-16 is observed. |
Customers also Viewed
Customers also Purchased
Citations for Mouse/Rat Wnt-5a Antibody (35)
Citations are publications that use Bio-Techne products. Selected citations for Mouse/Rat Wnt-5a Antibody include:
-
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot(2102), Ror family receptor tyrosine kinases regulate the maintenance of neural progenitor cells in the developing neocortex. J. Cell. Sci., 125(0):2017-29.
PMID: 22328498 -
Species: Rat
Sample Types: Whole Tissue
Applications: IHCAC van Vliet et al. (2021), Coordinated changes in the expression of Wnt pathway genes following human and rat peripheral nerve injury PLoS ONE, 16(4):e0249748.
PMID: 33848304 -
Species: Mouse
Sample Types: Cell Lysates
Applications: Western BlotCS Thomson et al. (2021), WNT5A inhibition alters the malignant peripheral nerve sheath tumor microenvironment and enhances tumor growth Oncogene, 40(24):4229-4241.
PMID: 34079083 -
Species: Mouse
Sample Types: Cell Culture Lysates
Applications: Western BlotW Kawarazaki et al. (2020), Salt causes aging-associated hypertension via vascular Wnt5a under Klotho deficiency J. Clin. Invest., 0(0).
PMID: 32597829 -
Species: Human
Sample Types: Whole Tissue
Applications: IHCCM Lund et al. (2020), Wnt5a expression and prognosis in stage II-III colon cancer Transl Oncol, 14(1):100892.
PMID: 33045677 -
Species: Mouse
Sample Types: Cell Lysates
Applications: ELISA DetectionS Ikezu et al. (2020), Inhibition of colony stimulating factor 1 receptor corrects maternal inflammation-induced microglial and synaptic dysfunction and behavioral abnormalities Mol. Psychiatry, 0(0).
PMID: 32071385 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-FrX Wu et al. (2019), Mesenchymal WNT-5A/5B Signaling Represses Lung Alveolar Epithelial Progenitors Cells, 8(10).
PMID: 31557955 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCC Han et al. (2018), Wnt5a Contributes to the Differentiation of Human Embryonic Stem Cells into Lentoid Bodies Through the Noncanonical Wnt/JNK Signaling Pathway Invest. Ophthalmol. Vis. Sci., 59(8):3449-3460.
PMID: 30025083 -
Species: Mouse
Sample Types: Cell Lysates
Applications: Western BlotJ Xu et al. (2018), Reduced Cdc42 Activity Compromises Hematopoiesis-Supportive Function Of Fanconi Anemia Mesenchymal Stromal Cells Stem Cells, 0(0).
PMID: 29377497 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotK Glaeser et al. (2018), ERAD-dependent control of the Wnt secretory factor Evi EMBO J., 37(4).
PMID: 29378775 -
Species: Human
Sample Types: Whole Cells
Applications: ICCT Skaria et al. (2017), Wnt5A/Ryk signaling critically affects barrier function in human vascular endothelial cells Cell Adh Migr, 11(1):24-38.
PMID: 27159116 -
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western BlotCM Chen et al. (2017), Wnt5a is essential for hippocampal dendritic maintenance and spatial learning and memory in adult mice Proc. Natl. Acad. Sci. U.S.A, 114(4):E619-E628.
PMID: 28069946 -
Species: Human
Sample Types: Whole Cells
Applications: IHC(2016), IL-4 Causes Hyperpermeability of Vascular Endothelial Cells through Wnt5A Signaling PLoS ONE, 11(5):e0156002.
PMID: 27214384 -
Species: Human
Sample Types: Whole Cells
Applications: Neutralization(2016), Butyrate and bioactive proteolytic form of Wnt-5a regulate colonic epithelial proliferation and spatial development Sci Rep, 6(0):32094.
PMID: 27561676 -
Species: Mouse
Sample Types: In Vivo
Applications: NeutralizationHoeke A Baarsma (2016), Noncanonical WNT-5A signaling impairs endogenous lung repair in COPD J. Exp. Med, 0(0).
PMID: 27979969 -
Species: Rat
Sample Types: Whole Tissue
Applications: IHC-PBruce F Kimler (2016), WINGLESS (WNT) signaling is a progesterone target for rat uterine stromal cell proliferation J. Endocrinol., 229(2):197-207.
PMID: 26975616 -
Species: N/A
Sample Types: Recombinant Protein
Applications: Bioassay(2016), Wnt-5a/Frizzled9 receptor signaling through the G?o/G?? complex regulates dendritic spine formation J Biol Chem, 0(0).
PMID: 27402827 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotEkstrom E et al. (2014), WNT5A induces release of exosomes containing pro-angiogenic and immunosuppressive factors from malignant melanoma cells. Mol Cancer, 13(0):88.
PMID: 24766647 -
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western BlotDawes L et al. (2014), Interactions between lens epithelial and fiber cells reveal an intrinsic self-assembly mechanism. Dev Biol, 385(2):291-303.
PMID: 24211762 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotKumawat K et al. (2014), TGF-beta-activated kinase 1 (TAK1) signaling regulates TGF-beta-induced WNT-5A expression in airway smooth muscle cells via Sp1 and beta-catenin. PLoS ONE, 9(4):e94801.
PMID: 24728340 -
Species: Mouse
Sample Types: Whole Cells
Applications: IHCFlorian M et al. (2013), A canonical to non-canonical Wnt signalling switch in haematopoietic stem-cell ageing. Nature, 503(7476):392-6.
PMID: 24141946 -
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot(2012), NMDA receptor activation stimulates transcription-independent rapid wnt5a protein synthesis via the MAPK signaling pathway. Mol Brain, 5(0):1.
PMID: 22217152 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotBitler BG et al. (2011), Wnt5a suppresses epithelial ovarian cancer by promoting cellular senescence. Cancer Res., 71(19):6184-94.
PMID: 21816908 -
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western BlotLi B et al. (2011), WNT5A Signaling Contributes to Abeta-Induced Neuroinflammation and Neurotoxicity. PLoS ONE, 6(8):e22920.
PMID: 21857966 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayGudjonsson JE et al. (2010), Evidence for altered Wnt signaling in psoriatic skin. J. Invest. Dermatol., 130(7):1849-59.
PMID: 20376066 -
Species: Mouse
Sample Types: Cell Lysates
Applications: Western BlotBodmer D et al. (2009), Wnt5a mediates nerve growth factor-dependent axonal branching and growth in developing sympathetic neurons. J. Neurosci., 29(23):7569-81.
PMID: 19515925 -
Species: Human
Sample Types: Whole Cells
Applications: ICCPereira C et al. (2008), Wnt5A/CaMKII signaling contributes to the inflammatory response of macrophages and is a target for the antiinflammatory action of activated protein C and interleukin-10. Arterioscler. Thromb. Vasc. Biol., 28(3):504-10.
PMID: 18174455 -
Species: Human
Sample Types: Whole Tissue
Applications: IHC-PChristman MA et al. (2008), Wnt5a is expressed in murine and human atherosclerotic lesions. Am. J. Physiol. Heart Circ. Physiol., 294(6):H2864-70.
PMID: 18456733 -
Species: Human
Sample Types: Tissue Homogenates
Applications: Western BlotDiarra D et al. (2007), Dickkopf-1 is a master regulator of joint remodeling. Nat. Med., 13(2):156-63.
PMID: 17237793 -
Species: Human
Sample Types: Whole Cells
Applications: NeutralizationBlumenthal A et al. (2006), The Wingless homolog WNT5A and its receptor Frizzled-5 regulate inflammatory responses of human mononuclear cells induced by microbial stimulation. Blood, 108(3):965-73.
PMID: 16601243 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCMasckauchan TN et al. (2006), Wnt5a signaling induces proliferation and survival of endothelial cells in vitro and expression of MMP-1 and Tie-2. Mol. Biol. Cell, 17(12):5163-72.
PMID: 17035633 -
Species: Mouse
Sample Types: Whole Cells
Applications: ICCCastelo-Branco G et al. (2005), Ventral midbrain glia express region-specific transcription factors and regulate dopaminergic neurogenesis through Wnt-5a secretion. Mol. Cell. Neurosci., 31(2):251-62.
PMID: 16243537 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCPolesskaya A et al. (2003), Wnt signaling induces the myogenic specification of resident CD45+ adult stem cells during muscle regeneration. Cell, 113(7):841-52.
PMID: 12837243 -
Species: Xenopus
Sample Types: Cell Culture Supernates
Applications: ImmunodepletionMurdoch B et al. (2003), Wnt-5A augments repopulating capacity and primitive hematopoietic development of human blood stem cells in vivo. Proc. Natl. Acad. Sci. U.S.A., 100(6):3422-7.
PMID: 12626754 -
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: ImmunodepletionAhumada A et al. (2002), Signaling of rat Frizzled-2 through phosphodiesterase and cyclic GMP. Science, 298(5600):2006-10.
PMID: 12471263
There are no citations that match your criteria.
Customer Reviews for Mouse/Rat Wnt-5a Antibody (1)
Customer Images
-
Application: WBSample Tested: B16-F1 mouse melanoma cell lineSpecies: MouseVerified Customer | Posted 02/19/2018Non-specific bands at 64kDa, actual band at 49KDa. Recommend to use 7.5% gel for better separation.
There are no reviews that match your criteria.