Mouse VE‑Cadherin Antibody
Catalog # MAB1002 | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Product Summary for Mouse VE‑Cadherin Antibody
Immunogen
Asp46-Gln592 (Gly67 del, Ile69Asp, Lys70Gln)
Accession # 2208309A
Specificity
Clonality
Host
Isotype
Scientific Data Images for Mouse VE‑Cadherin Antibody
Detection of VE‑Cadherin in bEnd.3 Mouse Cell Line by Flow Cytometry.
bEnd.3 mouse endothelioma cell line was stained with Rat Anti-Mouse VE-Cadherin Monoclonal Antibody (Catalog # MAB1002, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). View our protocol for Staining Membrane-associated Proteins.Applications for Mouse VE‑Cadherin Antibody
CyTOF-ready
Flow Cytometry
Sample: bEnd.3 mouse endothelioma cell line
Western Blot
Sample: Recombinant Mouse VE-Cadherin Fc Chimera (Catalog # 1002-VC)
Reviewed Applications
Read 1 review rated 4 using MAB1002 in the following applications:
Published Applications
Read 4 publications using MAB1002 in the following applications:
Formulation, Preparation and Storage
Purification
Reconstitution
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: VE-Cadherin
The cadherin (Ca++-dependent adherence) superfamily is a large group of membrane-associated glycoproteins that engage in homotypic, calcium-dependent, cell-cell adhesion events. The superfamily can be divided into at least five major subfamilies based on molecule gene structure, and/or extracellular (EC) and intracellular domains (1-4). Subfamilies include classical/type I, atypical/type II, and desmosomal-related cadherins (1-3). VE-Cadherin (vascular endothelial cadherin; also cadherin-5 and CD144) is a 125 kDa atypical/type II subfamily cadherin. Its subfamily classification is based principally on its genomic structure, as its physical structure is notably divergent from other type II subfamily members (2, 3). Mouse VE-Cadherin is synthesized as a 784 amino acid (aa) type I transmembrane (TM) preproprotein that contains a 24 aa signal peptide, a 21 aa prosequence, a 554 aa extracellular region (ECR), a 21 aa TM segment, and a 164 aa cytoplasmic domain (5, 6). The ECR contains five Ca++-binding cadherin domains that are approximately 105 aa in length. Cadherin domains are comprised of two beta‑sheets that are oriented like bread in a sandwich. Although complex, the N-terminal cadherin domain mediates trans interactions, while the internal domains contribute to cis multimerizations (7). Mouse VE-Cadherin ECR is 92%, 77%, and 73% aa identical to rat, human and porcine VE-Cadherin ECR, respectively. VE-Cadherin is involved in the maintenance of endothelial permeability. In this regard, VE-Cadherin does not initiate new blood vessel formation; it maintains it once formed. Thus, when VE‑Cadherin is downregulated, cells part and permeability increases (8). Notably, VEGF is known to promote vascular leakage, and apparently does so by inducing a beta‑arrestin-dependent endocytosis of VE-Cadherin (9). Part of this effect may be mediated by VE‑Cadherin itself which is reported to increase the membrane half-life of VEGF R2 (10). VE-Cadherin acts homotypically at sites of zonula adherens. On each expressing cell, it is proposed that VE-Cadherin first forms a trimer, which then dimerizes with a trimeric counterpart in-trans. Alternatively, two cis-dimers could act in-trans to generate homotypic binding (11). In addition to cell adhesion, VE‑Cadherin also is reported to mediate TGF-beta receptor assembly. When clustered, VE‑Cadherin enhances T betaRII/T betaRI assembly into an active receptor complex on endothelial cells (12). VE-Cadherin is expressed on endothelial cells, trophoblast cells, endothelial progenitor cells and embryonic hematopoietic cells (5, 8, 13, 14).
References
- Patel, S.D. et al. (2007) Curr. Opin. Struct. Biol. 13:690.
- Vestweber, D. (2008) Arterioscler. Thromb. Vasc. Biol. 28:223.
- Vincent, P.A. et al. (2004) Am. J. Physiol. Cell. Physiol. 286:C987.
- Cavallaro, U. et al. (2006) Exp. Cell Res. 312:659.
- Breier, G. et al. (1996) Blood 87:630.
- Huber, P. et al. (1996) Genomics 32:21.
- Pokutta, S. and W.I. Weis (2007) Annu. Rev. Cell Dev. Biol. 23:237.
- Crosby, C.V. et al. (2005) Blood 105:2771.
- Gavard, J. and J.S. Gutkind (2006) Nat. Cell Biol. 8:1223.
- Calera, M.R. et al. (2004) Exp. Cell Res. 300:248.
- Hewat, E.A. et al. (2007) J. Mol. Biol. 365:744.
- Rudini, N. et al. (2008) EMBO J. 27:993.
- Kogata, N. et al. (2006) Circ. Res. 98:897.
- Ema, M. et al. (2006) Blood 108:4018.
Long Name
Alternate Names
Gene Symbol
UniProt
Product Documents for Mouse VE‑Cadherin Antibody
Product Specific Notices for Mouse VE‑Cadherin Antibody
For research use only
Citations for Mouse VE‑Cadherin Antibody (4)
Citations are publications that use Bio-Techne products. Selected citations for Mouse VE‑Cadherin Antibody include:
-
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCYS Lee et al. (2021-09-09), Microbiota-derived lactate promotes hematopoiesis and erythropoiesis by inducing stem cell factor production from leptin receptor+ niche cells Experimental & Molecular Medicine, 2021-00(0).
PMID: 34497346 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCYI Rodriguez et al. (2020-11-13), Tumor Necrosis Factor Receptor-1 (p55) Deficiency Attenuates Tumor Growth and Intratumoral Angiogenesis and Stimulates CD8+ T Cell Function in Melanoma Cells, 2020-19(11).
PMID: 33202705 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-PLee YJ et al. (2010-01-26), Variable inhibition of thrombospondin 1 against liver and lung metastases through differential activation of metalloproteinase ADAMTS1. Cancer Res., 2010-070(3):948-56.
PMID: 20103648 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-PHooper AT et al. (2009-03-06), Engraftment and reconstitution of hematopoiesis is dependent on VEGFR2-mediated regeneration of sinusoidal endothelial cells. Cell Stem Cell, 2009-04(3):263-74.
PMID: 19265665
There are no citations that match your criteria.
Customer Reviews for Mouse VE‑Cadherin Antibody (1)
Customer Images
-
Application: Immunocytochemistry/ImmunofluorescenceSample Tested: Intestinal villiSpecies: MouseVerified Customer | Posted 07/11/2019
There are no reviews that match your criteria.
Reconstitution Calculator
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.