Detects mouse Tryptase beta‑1/MCP‑7/Mcpt7 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross‑reactivity with recombinant human (rh) TPSB2 is observed, 5% cross-reactivity with rhTPS1 is observed, and less than 1% cross-reactivity with rhTPSG1 and recombinant mouse MCPT1 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Mouse Tryptase beta-1/MCP-7/Mcpt7 Antibody

Application

Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Mouse Tryptase beta-1/MCP-7/Mcpt7 (Catalog # 1937-SE), see our available Western blot detection antibodies

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse Tryptase beta-1/MCP-7/Mcpt7 (Catalog # 1937-SE)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Tryptase beta-1/MCP-7

Tryptase beta-1 is a serine protease with trypsin-like activity, which is sometimes also referred to as Mast Cell Protease 7 (1). It is stored in the secretory granules of mouse mast cells (2). It exhibits anticoagulant activity due to its ability to degrade fibrinogen in the presence of the diverse array of protease inhibitors in plasma (3). The deduced amino acid sequence for mouse Tryptase beta-1 consists of a signal peptide (residues 1‑18), a pro region (residues 19‑28) and the mature chain (residue 29‑273). The mature chain was expressed and purified.

References

McNeil, H.P. et al. (1992) Proc. Natl. Acad. Sci. USA 89:11174.
Matsumoto, R et al. (1995) J. Biol. Chem. 270:19524.
Huang, C. et al. (1997) J. Biol. Chem. 272:31885.

Long Name

Mast Cell Protease 7

Alternate Names

Mast Cell Tryptase, MCP-7, Mcp7, Mcpt7, TPSB1, TRYB1, Tryptase beta1

Entrez Gene IDs

100503895 (Mouse)

Gene Symbol

TPSAB1

UniProt

Q02844

Additional Tryptase beta-1/MCP-7 Products

Product Documents for Mouse Tryptase beta-1/MCP-7/Mcpt7 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse Tryptase beta-1/MCP-7/Mcpt7 Antibody

For research use only

Citations
Reviews

Mouse Tryptase beta-1/MCP-7/Mcpt7 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF1937

Key Product Details

Species Reactivity

Validated:

Cited:

Applications