Mouse Thrombospondin-1 Antibody

Name: Mouse Thrombospondin-1 Antibody
Brand: R&D Systems
SKU: MAB78591

R&D Systems, Inc. a Bio-Techne Brand

Catalog # MAB78591

Product Datasheet / COA / SDS

Recombinant Monoclonal Antibody.

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Key Product Details

Species Reactivity:	Mouse
Immunogen:	Mouse myeloma cell line NS0-derived recombinant mouse Thrombospondin-1 Asn19-Ser1171 Accession # P35441
Applications:	ELISA
Label:	Unconjugated
Source:	Recombinant Monoclonal Rat IgG_2B Clone # 899642R
Specificity:	Detects mouse Thrombospondin-1 in direct ELISAs.

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Applications for Mouse Thrombospondin-1 Antibody

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Sample

ELISA

This antibody functions as an ELISA capture antibody when paired with Rat Anti-Mouse Thrombospondin‑1 Monoclonal Antibody (Catalog # MAB7859).

This product is intended for assay development on various assay platforms requiring antibody pairs.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

Purification	Protein A or G purified from cell culture supernatant
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Thrombospondin-1

Thrombospondin‑1 (TSP‑1) is a 150‑180 kDa calcium‑sensitive protein that is secreted as a disulfide‑linked homotrimer. Within the Thrombospondin family, TSP‑2 is also homotrimeric, while TSP-3, -4, and -5/COMP are pentameric. TSP‑1 regulates a wide range of cellular functions including their interactions with other cells and with the extracellular matrix (ECM). TSP-1 contains an N‑terminal Laminin G‑like globular domain, an extended central region with one vWF‑C domain, 3 TSP type 1 repeats, 3 EGF‑like domains, and 8 TSP type‑3 repeats, and a globular C‑terminal lectin domain. Distinct regions of TSP‑1 have been associated with binding to particular ECM or cellular molecules (1, 2). Mature mouse TSP‑1 shares 95% and 99% amino acid sequence identity with human and rat TSP‑1, respectively. TSP‑1 counteracts the angiogenic, hypotensive, and anti‑thrombotic effects of nitric oxide (NO) (3). It binds and neutralizes VEGF, blocks VEGF R2 signaling on vascular endothelial cells (EC), and destabilizes adhesive contacts between EC (4‑6). TSP‑1’s actions on vascular smooth muscle cells support arterial tone and blood pressure in the presence of vasodilatory stimulation (7). It is released from activated platelets and enhances platelet aggregation and adhesion (8). TSP-1 also plays an important role in wound repair and tissue fibrosis by binding latent TGF‑beta and inducing release of the active cytokine from the latency associated peptide (LAP) (9, 10). TSP‑1 dampens adaptive immune responses by inducing the differentiation of regulatory T cells and inhibiting TCR signaling (11, 12). In the nervous system, it promotes excitatory synapse formation (13, 14) and supports the integration of neural progenitor cells into the olfactory bulb (15). In addition, TSP‑1 is released by apoptotic cells and promotes macrophage-mediated debris clearance (16).

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References	Murphy-Ullrich, J.E. and R.V. Iozzo (2012) Matrix Biol. 31:152. Laherty, C.D. et al. (1992) J. Biol. Chem. 267:3274. Roberts, D.D. et al. (2012) Matrix Biol. 31:162. Gupta, K. et al. (1999) Angiogenesis 3:147. Kaur, S. et al. (2010) J. Biol. Chem. 285:38923. Garg, P. et al. (2011) Am. J. Physiol. Lung Cell. Mol. Physiol. 301:L79. Isenberg, J.S. et al. (2009) Matrix Biol. 28:110. Isenberg, J.S. et al. (2008) Blood 111:613. Schultz-Cherry, S. et al. (1994) J. Biol. Chem. 269:26775. Sweetwyne, M.T. and J.E. Murphy-Ullrich (2012) Matrix Biol. 31:178. Grimbert, P. et al. (2006) J. Immunol. 177:3534. Kaur, S. et al. (2011) J. Biol. Chem. 286:14991. Xu, J. et al. (2010) Nat. Neurosci. 13:22. Eroglu, C. et al. (2009) Cell 139:380. Blake, S.M. et al. (2008) EMBO J. 27:3069. Moodley, Y. et al. (2003) Am. J. Pathol. 162:771.
Entrez Gene IDs	7057 (Human); 21825 (Mouse)
Alternate Names	THBS, THBS-1, THBS1, TSP-1, TSP1thrombospondin-1, TSPTSP-1, Thrombospondin-1, Thrombospondin1, thrombospondin 1, thrombospondin-1p180

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Mouse Thrombospondin-1 Antibody

R&D Systems, Inc. a Bio-Techne Brand

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Background: Thrombospondin-1

Citations for Mouse Thrombospondin-1 Antibody

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