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Mouse TDRD1 Antibody

Catalog # AF7320 | R&D Systems, Inc. a Bio-Techne Brand
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AF7320
AF7320-SP

Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

Product Summary for Mouse TDRD1 Antibody

Immunogen

E. coli-derived recombinant mouse TDRD1
Asn831-Lys960
Accession # Q99MV1

Specificity

Detects mouse TDRD1 in direct ELISAs and Western blots. In direct ELISAs, approximately 60% cross-reactivity with recombinant human TDRD1 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Mouse TDRD1 Antibody

Detection of Mouse TDRD1 antibody by Western Blot.

Detection of Mouse TDRD1 by Western Blot.

Western blot shows lysates of mouse testis tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse TDRD1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7320) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for TDRD1 at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Mouse TDRD1 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Mouse testis tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TDRD1

TDRD1 (Tudor domain containing protein 1; also MTR-1) is a 130-145 kDa member of the Tudor family of proteins. It is expressed in spermatocytes and spermatids, and forms part of an RNA-silencing complex that regulates gene expression. TDRD1, through its Tudor and MYND domains, binds methylated PIWI (P-element-induced wimpy testis) protein and PIWI-interacting RNA loading factor, respectively. This serves to promote the recruitment of germline-specific small RNAs into a PIWI ribonucleoprotein complex. Mouse TDRD1 is 1172 aa in length. It contains one MYND-type Zn finger region (aa 163-199), and four methyl-binding Tudor domains (aa 307-1032). Over aa 831-960, mouse TDRD1 shares 96% and 66% aa identity with rat and human TDRD1, respectively.

Long Name

Tudor Domain Containing 1

Alternate Names

CT41.1

Entrez Gene IDs

56165 (Human); 83561 (Mouse); 292129 (Rat)

Gene Symbol

TDRD1

UniProt

Product Documents for Mouse TDRD1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse TDRD1 Antibody

For research use only

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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