Mouse Semaphorin 4A Alexa Fluor™ Plus 488-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB8166AFP488

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FAB8166AFP488-100UG
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Conjugate
Catalog #
Unconjugated
MAB8166
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Alexa Fluor 350
FAB8166U
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Alexa Fluor 405
FAB8166V
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Alexa Fluor 488
FAB8166G
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Alexa Fluor 532
FAB8166X
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Alexa Fluor 594
FAB8166T
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Alexa Fluor 647
FAB8166R
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Alexa Fluor 700
FAB8166N
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Alexa Fluor 750
FAB8166S
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Alexa Fluor Plus 405
FAB8166AFP405
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Alexa Fluor Plus 555
FAB8166AFP555
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Alexa Fluor Plus 594
FAB8166AFP594
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Alexa Fluor Plus 647
FAB8166AFP647
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Alexa Fluor Plus 680
FAB8166AFP680
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Allophycocyanin
FAB8166A
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Allophycocyanin/Cy7
FAB8166APCCY7
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Biotin
FAB8166B
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CoraFluor 1
FAB8166CL1
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DyLight 350
FAB8166D
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DyLight 405
FAB8166E
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DyLight 488
FAB8166K
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DyLight 550
FAB8166L
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DyLight 594
FAB8166M
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DyLight 650
FAB8166W
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DyLight 680
FAB8166Y
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DyLight 755
FAB8166Z
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FITC
FAB8166F
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HRP
FAB8166H
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Janelia Fluor 525
FAB8166JF525
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Janelia Fluor 549
FAB8166I
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Janelia Fluor 646
FAB8166J
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Janelia Fluor 669
FAB8166JF669
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mFluor Violet 450 SE
FAB8166MFV450
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mFluor Violet 500 SE
FAB8166MFV500
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mFluor Violet 610 SE
FAB8166MFV610
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PE/Atto594
FAB8166PEATT594
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PE/Cy5.5
FAB8166PECY55
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PE/Cy7
FAB8166PECY7
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PerCP
FAB8166C
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Key Product Details

Species Reactivity

Mouse

Applications

Neutralization, Flow Cytometry, CyTOF-ready

Label

Alexa Fluor Plus 488 (Excitation = 493 nm, Emission = 518 nm)

Antibody Source

Monoclonal Rat IgG1 Clone # 757129

View all Semaphorin 4A Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Semaphorin 4A

Specificity

Detects mouse Semaphorin 4A in ELISA. Stains HEK293 cells transfected with mouse Semaphorin 4A by Flow Cytometry, but does not stain untrasfected cells.

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Applications

Application
Recommended Usage

CyTOF-ready

Optimal dilution of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilution of this antibody should be experimentally determined.

Neutralization

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Semaphorin 4A

Semaphorin 4A (Sema4A, previously semB) is type I transmembrane protein that is a Class 4 transmembrane Semaphorin with activity in the immune and nervous systems. It is expressed by dendritic cells, monocytes, T cells, B cells, astrocytes and oligodendrocytes, plus visceral smooth muscle and bronchial epithelial cells. The 760 amino acid (aa) mouse Sema4A contains a 650 aa extracellular domain (ECD, aa 33-682) with Sema, PSI and C2-type immunoglobulin domains. It interacts with B and D1 Plexins and lymphocyte TIM-2, enhancing dendritic cell-mediated T cell priming and Th1 responses. Sema4A interaction with endothelial cell plexin-D1 downregulates the actions of VEGF. Mutations of Sema4A are associated with retinitis pigmentosa and cone rod dystrophy in humans. Mouse Sema4A ECD shares 87% and 94% aa sequence identity with human and rat Sema4A, respectively, and shares approximately 35% aa identity with other Sema4 family members. Using this antibody, Sema4A has been shown to interact with Neuropilin-1 in vitro and in vivo to potentiate Treg cell function and survival.

References

  1. Delgoffe, G.M. et al (2013) Nature 501:252.

Alternate Names

CORD10, SEMA4A, SEMAB, SEMB

Entrez Gene IDs

64218 (Human); 20351 (Mouse); 310630 (Rat)

Gene Symbol

SEMA4A

UniProt

Q62178

Additional Semaphorin 4A Products

Product Documents

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

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