Mouse Sca-1/Ly6 APC-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB1226A
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Flow Cytometry
Cited:
Flow Cytometry
Label
Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)
Antibody Source
Monoclonal Rat IgG2A Clone # 177228
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Sca‑1/Ly6 C-terminally truncated Ly-6E allele
Leu27-Gly119
Accession # CAA28351
Leu27-Gly119
Accession # CAA28351
Specificity
Detects mouse Sca‑1/Ly6 in direct ELISAs.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Mouse Sca-1/Ly6 APC-conjugated Antibody
Detection of Sca‑1/Ly6 in Mouse Splenocytes by Flow Cytometry.
Mouse splenocytes gated on hematopoietic lineage negative cells were stained with Rat Anti-Mouse CD117/c-kit PE-conjugated Monoclonal Antibody (Catalog # FAB1356P) and either (A) Rat Anti-Mouse Sca-1/Ly6 APC-conjugated Monoclonal Antibody (Catalog # FAB1226A) or (B) Rat IgG2AAllophycocyanin Isotype Control (Catalog # IC006A). View our protocol for Staining Membrane-associated Proteins.Applications for Mouse Sca-1/Ly6 APC-conjugated Antibody
Application
Recommended Usage
Flow Cytometry
10 µL/106 cells
Sample: Mouse splenocytes gated on hematopoietic lineage negative cells
Sample: Mouse splenocytes gated on hematopoietic lineage negative cells
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectra Viewer
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Sca-1/Ly6
References
- Van de Rijn, M. et al. (1989) Proc. Natl. Acad. Sci. USA 86:4634.
- Spangrude, G.I. et al. (1988) Science 241:58.
- Spangrude, G.I. et al. (1993) Blood 82:3327.
- Morrison, S.J. et al. (1995) Proc. Natl. Acad. Sci. USA 92:10302.
- Kawamoto, H. et al. (1997) Int. Immunol. 9:1011.
- Yamamoto, Y. et al. (1996, Blood 88:445.
- Morrison, S.J. et al. (1997) Proc. Natl. Acad. Sci. USA 94:1908.
- Welm, B.E. et al. (2002) Dev. Biol. 245:42.
- Codias, E.K. et al. (1990) J. Immunol. 144:2197.
- Malek, T.R. et al. (1986) J. Exp. Med. 164:709.
- Codias, E.K. et al. (1990) J. Immunol. 145:1407.
- Flood, P.M. et al. (1990) J. Exp. Med. 172:115.
Long Name
Stem Cell Antigen-1/Lymphocyte Antigen 6
Alternate Names
Ly-6, Ly6, Sca1
Entrez Gene IDs
110454 (Mouse)
Gene Symbol
LY6A
UniProt
Additional Sca-1/Ly6 Products
Product Documents for Mouse Sca-1/Ly6 APC-conjugated Antibody
Product Specific Notices for Mouse Sca-1/Ly6 APC-conjugated Antibody
For research use only
Loading...
Loading...
Loading...
Loading...
Loading...