Mouse/Rat CD47 N-terminal IgV-like Extracellular Domain Alexa Fluor™ Plus 405-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # AF1866AFP405

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AF1866AFP405-100UG
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Key Product Details

Species Reactivity

Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Label

Alexa Fluor Plus 405 (Excitation = 404 nm, Emission = 455 nm)

Antibody Source

Polyclonal Goat IgG

View all CD47 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse CD47 N-terminal IgV-like

Specificity

Detects mouse CD47 N-terminal IgV-like in direct ELISAs and Western blots. In direct ELISAs, less than 15% cross-reactivity with recombinant human CD47 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications

Application
Recommended Usage

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: CD47

CD47, also known as Integrin‑Associated Protein (IAP) and OA3, is a 40‑60 kDa variably glycosylated atypical member of the immunoglobulin superfamily (1, 2). Mouse CD47 is an integral membrane protein that consists of a 122 amino acid (aa) extracellular domain (ECD) with a single Ig‑like domain, five membrane-spanning regions with short intervening loops, and a 16 aa C‑terminal cytoplasmic tail (3). Alternate splicing of mouse CD47 generates an additional isoform with an insertion of 21 aa following the Ig‑like domain (3). Within the N‑terminal ECD, mouse CD47 shares 63% and 84% aa sequence identity with human and rat CD47, respectively. A portion of the N‑terminal ECD can by shed from smooth muscle cells by MMP-2‑mediated proteolysis (4). The ubiquitously expressed CD47 binds to SIRP family members on macrophages, neutrophils, and T cells (5, 6). These interactions prevent macrophage‑mediated clearance of healthy CD47-expressing cells and promote immune cell transmigration across the vascular endothelium (5‑8). CD47 associates in cis with Fas on T cells and enhances Fas‑mediated apoptosis; its ligation promotes T cell anergy and dampens Th1 immune responses (9‑11). CD47 also associates in cis with Integrins alpha4 beta1, alphaV beta3, alpha2b beta3, and alpha2 beta1 which can positively or negatively modulate Integrin-mediated function (2, 12). In the vasculature, CD47 binding by Thrombospondin‑1 inhibits the angiogenic and vasorelaxant effects of nitric oxide (2, 13, 14). On dendritic cells and myeloma cells, CD47 ligation by TSP‑1 induces giant cell formation and osteoclast differentiation (15).

References

  1. Sarfati, M. et al. (2009) Curr. Drug Targ. 9:842.
  2. Isenberg, J.S. et al. (2008) Arterioscler. Thromb. Vasc. Biol. 28:615.
  3. Lindberg, F.P. et al. (1993) J. Cell Biol. 123:485.
  4. Maile, L.A. et al. (2008) Mol. Endocrinol. 22:1226.
  5. Oldenborg, P.-A. et al. (2000) Science 288:2051.
  6. Liu, Y. et al. (2002) J. Biol. Chem. 277:10028.
  7. Stefanidakis, M. et al. (2008) Blood 112:1280.
  8. de Vries, H.E. et al. (2002) J. Immunol. 168:5832.
  9. Manna, P.P. et al. (2005) J. Biol. Chem. 280:29637.
  10. Avice, M.-N. et al. (2001) J. Immunol. 167:2459.
  11. Bouguermouh, S. et al. (2008) J. Immunol. 180:8073.
  12. Barazi, H.O. et al. (2002) J. Biol. Chem. 277:42859.
  13. Isenberg, J.S. et al. (2009) Nitric Oxide 21:52.
  14. Isenberg, J.S. et al. (2009) Matrix Biol. 28:110.
  15. Kukreja, A. et al. (2009) Blood 114:3413.

Alternate Names

CD47, IAP, MER6, OA3

Entrez Gene IDs

961 (Human); 16423 (Mouse); 397042 (Porcine); 478552 (Canine); 102139846 (Cynomolgus Monkey)

Gene Symbol

CD47

UniProt

NP_034711

Additional CD47 Products

Product Documents

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

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