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Mouse/Rat CD31/PECAM‑1 APC‑conjugated Antibody

Catalog # FAB3628A | R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Species Reactivity

Mouse, Rat

Applications

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Polyclonal Goat IgG

Product Summary for Mouse/Rat CD31/PECAM‑1 APC‑conjugated Antibody

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse CD31/PECAM-1
Glu18-Lys590
Accession # Q08481

Specificity

Detects mouse CD31/PECAM-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human CD31 and recombinant porcine CD31 is observed. Detects mouse CD31 and rat CD31 in flow cytometry.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse/Rat CD31/PECAM‑1 APC‑conjugated Antibody

Detection of CD31/PECAM-1 antibody in Mouse Splenocytes antibody by Flow Cytometry.

Detection of CD31/PECAM‑1 in Mouse Splenocytes by Flow Cytometry.

Mouse splenocytes were stained with Goat Anti-Mouse/Rat CD31/PECAM-1 APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB3628A, filled histogram) or isotype control antibody (Catalog # IC108A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of CD31/PECAM-1 antibody in Rat Splenocytes antibody by Flow Cytometry.

Detection of CD31/PECAM‑1 in Rat Splenocytes by Flow Cytometry.

Rat splenocytes were stained with Goat Anti-Mouse/Rat CD31/PECAM-1 APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB3628A, filled histogram) or isotype control antibody (Catalog # IC108A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of Mouse CD31/PECAM-1 by Flow Cytometry

Detection of Mouse CD31/PECAM-1 by Flow Cytometry

CD31hiEmcnhi vessel and bone formation decreased during ageing but increased in bone regeneration. A, Representative images of CD31 (green), EMCN (red) and Osterix (white) immunostaining. Scale bar, 100 μm. G, growth plate. B, Quantification of CD31 and EMCN positive vessel volume in distal femora. C, Quantitative analysis of Osterix‐positive (OSX+) osteoprogenitors in distal femora. D and E, FACS analysis dot plot (D) and quantification (E) of CD31hiEMCNhi ECs. F, Representative images of CD31 (green) and EMCN (red) immunostaining in bone regeneration area after femoral trabecular bone ablation. Nuclei, DAPI (blue). Scale bar, 100 μm. G and H, Representative μCT images (G) and quantitative μCT analysis (H) of bone regeneration after femoral trabecular bone ablation. Selected areas for the measurements of bone volume (BV)/tissue volume (TV) were indicated with a yellow square. Data are shown as mean ± SD, (n = 6 in B, C, E and H). *P 

Applications for Mouse/Rat CD31/PECAM‑1 APC‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Mouse splenocytes and rat splenocytes
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using FAB3628A in the following applications:

Published Applications

Read 6 publications using FAB3628A in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD31/PECAM-1

PECAM-1 (Platelet-Endothelial Cell Adhesion Molecule-1), also known as CD31, is a 130 kDa type I transmembrane glycoprotein adhesion molecule in the immunoglobulin superfamily (1, 2). Expression is restricted to cells involved in circulation, especially endothelial cells, platelets, monocytes, neutrophils and lymphocyte subsets. PECAM-1 is concentrated at cell-cell junctions and is required for Transendothelial Migration (TEM) (1-3). The Extracellular Domain (ECD) of PECAM-1 has ten potential N-linked glycosylation sites and six C2-type Ig-like domains, the first of which is critical for adhesion and extravasation (3, 4). The cytoplasmic domain contains Immunoregulatory Tyrosine-based Inhibitory and Switch Motifs (ITIM, ITSM) that mediate both inhibition and activation via phosphotyrosine-mediated engagement of SH2-containing signaling molecules (1, 5). Metalloproteinase-mediated ectodomain shedding occurs during apoptosis (6) but increased serum PECAM-1 ectodomain in HIV and active multiple sclerosis occurs independent of apoptosis (7, 8). In humans, expression of six isoforms with exon deletions in the cytoplasmic domain is tissue- and stage-specific, but full-length PECAM-1 is predominant. A form lacking the ITSM predominates in mouse (9). Mouse PECAM-1 ECD shows 77%, 63%, 63%, 63%, and 61% amino acid (aa) identity with rat, human, canine, porcine, and bovine PECAM-1, respectively. PECAM-1 participates with other adhesion molecules in some functions, but is the critical molecule for TEM. Homotypic PECAM-1 adhesion in trans, combined with cycling of PECAM-1 to and from surface-connected endothelial cell vesicles, leads leukocytes across endothelial tight junctions (3, 10). Homotypic adhesion and signaling functions also strongly suppress mitochondria-dependent apoptosis (11). In platelets, PECAM-1 is necessary for limiting thrombus formation (12) and promoting integrin-mediated clot retraction and platelet spreading (13), but mechanisms for these phenomena are unclear. PECAM-/- mice are deficient in chemokine-mediated chemotaxis (14).

References

  1. Ilan, N. and J.A. Madri (2003) Curr. Opin. Cell Biol. 15:515.
  2. Xie, Y. and W.A. Muller (1993) Proc. Natl. Acad. Sci. USA 90:5569.
  3. Liao, F. et al. (1997) J. Exp. Med. 185:1349.
  4. Nakada, M.T. et al. (2000) J. Immunol. 164:452.
  5. Chemnitz, J.M. et al. (2004) J. Immunol. 173:945.
  6. Ilan, N. et al. (2001) FASEB J. 15:362.
  7. Eugenin, E.A. et al. (2006) J. Leukoc. Biol. 79:444.
  8. Losy, J. et al. (1999) J. Neuroimmunol. 99:169.
  9. Wang, Y. et al. (2003) Am. J. Physiol. Heart Circ. Physiol. 284:H1008.
  10. Mamdouh, Z. et al. (2003) Nature 421:748.
  11. Gao, C. et al. (2003) Blood 102:169.
  12. Falati, S. et al. (2006) Blood 107:535.
  13. Wee, J.L. and D.E. Jackson (2005) Blood 106:3816.
  14. Wu, Y. et al. (2005) J. Immunol. 175:3484.

Long Name

Platelet Endothelial Cell Adhesion Molecule 1

Alternate Names

CD31, EndoCAM, PECA1, PECAM-1, PECAM1

Entrez Gene IDs

5175 (Human); 18613 (Mouse); 29583 (Rat)

Gene Symbol

PECAM1

UniProt

Product Documents for Mouse/Rat CD31/PECAM‑1 APC‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse/Rat CD31/PECAM‑1 APC‑conjugated Antibody

For research use only

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