Mouse PU.1/Spi-1 Alexa Fluor™ Plus 405-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF7124AFP405
Conjugate
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
Western Blot
Label
Alexa Fluor Plus 405 (Excitation = 404 nm, Emission = 455 nm)
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant mouse PU.1/Spi-1
Specificity
Detects mouse PU.1/Spi-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant human Spi-1 is observed, and less than 1% cross-reactivity with recombinant mouse Spi-B is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications
Application
Recommended Usage
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: PU.1/Spi-1
Spi1 (SFFV proviral integration 1 protein; also PU.1 and Sfpi1) is a 37-41 kDa member of the ets family of transcription factors. It is both an RNA and DNA binding protein that is found in hematopoietic cells such as B cells, neutrophils, macrophages and dendritic cells (DC). Spi1 can act as both a transcriptional activator and repressor. In DC, Spi1 promotes the expression of CD80, CD86 and CD11b, while in proerythroblasts, it blocks GATA-1 induced transcriptional activation. Spi1 binds to DNA as a monomer, and interacts with multiple factors such as Runx-1, IRF8, GATA-1 and c-Jun. Mouse Spi1 is 272 amino acids (aa) in length. It contains a transactivation region (aa 7-117), a PEST domain (aa 118-164), and a DNA-binding domain (aa 165-266). Phosphorylation on Ser41, 142 and 148 increases activity. There is one alternative start site at Met7. Over aa 1-169, mouse Spi1 shares 88% and 81% aa identity with rat and human Spi1, respectively.
Long Name
Hematopoietic transcription factor PU.1
Alternate Names
OF, SFPI1, Spi-1, SPI-A, SPI1
Gene Symbol
SPI1
UniProt
Additional PU.1/Spi-1 Products
Product Specific Notices
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
For research use only
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