Mouse Prostasin/Prss8 Alexa Fluor™ Plus 488-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF2968AFP488
Conjugate
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
Western Blot, ELISA Capture (Matched Antibody Pair)
Label
Alexa Fluor Plus 488 (Excitation = 493 nm, Emission = 518 nm)
Antibody Source
Polyclonal Goat IgG
Product Specifications
Specificity
Detects mouse Prostasin/Prss8 in ELISAs and Western blots. In sandwich immunoassays, less than 0.2% cross-reactivity is observed with recombinant human Prostasin.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications
Application
Recommended Usage
ELISA Capture (Matched Antibody Pair)
Optimal dilution of this antibody should be experimentally determined.
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Background: Prostasin/Prss8
Prostasin encoded by the Prss8 gene, also known as channel activating protease 1, is a serine protease with a trypsin-like substrate specificity (1, 2). The preproenzyme possesses a C-terminal membrane-spanning domain that can be proteolytically processed to generate a secreted form of the enzyme. The secreted form of prostasin has been purified from seminal fluid (2). Prostasin is highly expressed in the prostate gland, and is expressed at lower levels in the lung, kidney, liver, salivary gland, and pancreas (3). The peptidase activity of prostasin is involved in the regulation of epithelial sodium channels (4, 5). Secreted from NS0 cells as the active form, the purified recombinant mouse (rm) Prostasin most likely consists of a disulfide bond-linked two chains with N-terminal sequences of I45TGGGSAKPG and A30DGTE, respectively (3). The enzymatic activity of rmProstasin is effectively inhibited by rmHAI-1 and rmHAI‑2B (Catalog # http://www.rndsystems.com/product_results.aspx?k=1141-PI">1141-PI and http://www.rndsystems.com/product_results.aspx?k=1107-PI">1107-PI).
References
- Vuagniaux, G. et al. (2000) J. Am. Soc. Nephrol. 11:828.
- Yu J.X. et al. (1994) J. Biol. Chem. 269:18843.
- Yu J.X. et al. (1995) J. Biol. Chem. 270:13483.
- Caldwell R.A. et al. (2004) Am. J. Physiol. Cell Physiol. 286:C190.
- Tong Z. et al. (2004) Am. J. Physiol. Lung Cell Mol. Physiol. 287:L928.
Alternate Names
Prss8
Gene Symbol
PRSS8
UniProt
Additional Prostasin/Prss8 Products
Product Specific Notices
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
For research use only
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