Mouse Podoplanin Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB11608
Key Product Details
| Species Reactivity: | Mouse |
| Immunogen: |
Mouse myeloma cell line, NS0-derived mouse Podoplanin Gln21-Lys133 Accession # Q62011 |
| Applications: | Immunohistochemistry, Western Blot |
| Label: | Unconjugated |
| Source: | Monoclonal Rat IgG1 Clone # 1090106 |
| Specificity: |
Detects recombinant mouse Podoplanin in Direct ELISA |
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Western BlotDetection of Mouse Podoplanin by Western Blot. Western Blot shows lysates of mouse lung tissue. PVDF membrane was probed with 1 µg/ml of Rat Anti-Mouse Podoplanin Monoclonal Antibody (Catalog # MAB11608) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Podoplanin at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1. |
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ImmunohistochemistryDetection of Podoplanin in Mouse Kidney. Podoplanin was detected in immersion fixed paraffin-embedded sections of mouse kidney using Rat Anti-Mouse Podoplanin Monoclonal Antibody (Catalog # mab11608) at 5 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using the HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005) and counterstained with hematoxylin (blue). Specific staining was localized to the cell membrane of podocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
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ImmunohistochemistryDetection of Podpalanin in Mouse Lung. Podoplanin was detected in immersion fixed paraffin-embedded sections of mouse lung using Rat Anti-Mouse Podoplanin Monoclonal Antibody (Catalog # mab11608) at 5 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using the HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005) and counterstained with hematoxylin (blue). Specific staining was localized to the cell membrane of alveoli. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
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