Mouse PD‑1 Antibody

Catalog # AF1021 | R&D Systems, Inc. a Bio-Techne Brand
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AF1021
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Key Product Details

Species Reactivity

Mouse

Applications

CyTOF-ready, Flow Cytometry, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Product Summary for Mouse PD‑1 Antibody

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse PD-1
Leu25-Gln167
Accession # Q02242

Specificity

Detects mouse PD-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human PD‑1 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse PD‑1 Antibody

Detection of Mouse PD-1 antibody by Western Blot.

Detection of Mouse PD‑1 by Western Blot.

Western blot shows lysates of EL-4 mouse lymphoblast cell line and CTLL-2 mouse cytotoxic T cell line (negative control). PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Mouse PD-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1021) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for PD-1 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Mouse PD-1 antibody by Western Blot.

Detection of Mouse PD‑1 by Western Blot.

Western blot shows lysates of 293T human embryonic kidney cell line mock transfected or transfected with mouse PD-1. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Mouse PD-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1021) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for PD-1 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
PD‑1 antibody in Mouse Thymus by Immunohistochemistry (IHC-Fr).

PD‑1 in Mouse Thymus.

PD‑1 was detected in perfusion fixed frozen sections of mouse thymus using 15 µg/mL Mouse PD‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1021) overnight at 4 °C. Tissue was stained (red) and counterstained (green). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
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Applications for Mouse PD‑1 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: Mouse splenocytes treated with PHA

Immunohistochemistry

5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse thymus and mouse spleen, and perfusion fixed paraffin-embedded sections of mouse thymus

Western Blot

0.2 µg/mL
Sample: EL‑4 mouse lymphoblast cell line and 293T human embryonic kidney cell line transfected with mouse PD-1
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 5 reviews rated 4.6 using AF1021 in the following applications:

Formulation, Preparation and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PD-1

Programmed Death-1 (PD-1) is type I transmembrane protein belonging to the CD28/CTLA-4 family of immunoreceptors that mediate signals for regulating immune responses (1). Other members of this family include CD28, CTLA-4, and ICOS (2-4). PD-1 is most closely related to CTLA-4 and shares approximately 24% amino acid (aa) sequence identity. The mouse PD-1 gene encodes a 288 aa protein with a putative 20 aa signal peptide, a 149 aa extracellular region with one immunoglobulin-like V-type domain, a 21 aa transmembrane domain, and a 98 aa cytoplasmic region. The cytoplasmic tail contains two tyrosine residues that form the immunoreceptor tyrosine-based inhibitory motif (ITIM) and immunoreceptor tyrosine-based switch motif (ITSM) that are important in mediating PD-1 signaling. Mouse and human PD-1 share approximately 69% aa sequence identity. Two B7 family proteins, PD-L1 (also called B7-H1) and PD-L2, have been identified as PD-1 ligands (5, 6). PD-1 is expressed on activated T cells, B cells, myeloid cells, and on a subset of thymocytes. PD-1 deficient mice have a defect in peripheral tolerance and spontaneously develop autoimmune diseases. Binding of PD-1 to PD-L1 or PD-L2 results in the inhibition of TCR-mediated proliferation and cytokine production as well as BCR-mediated signaling. PD-1 likely has an inhibitory role in regulating immune responses (1-4).

References

  1. Ishida, Y. et al. (1992) EMBO J. 11:3887.
  2. Sharpe, A.H. and G.J. Freeman (2002) Nat. Rev. Immunol. 2:116.
  3. Coyle, A. and J. Gutierrez-Ramos (2001) Nat. Immunol. 2:203.
  4. Nishimura, H. and T. Honjo (2001) Trends in Immunol. 22:265.
  5. Latchman Y. et al. (2001) Nature Immun. 2:261.
  6. Tamura, H. et al. (2001) Blood 97:1809.

Long Name

Programmed Death-1

Alternate Names

CD279, PD1, PDCD1, SLEB2

Entrez Gene IDs

486213 (Canine); 102123659 (Cynomolgus Monkey); 5133 (Human); 18566 (Mouse); 100533201 (Porcine); 301626 (Rat)

Gene Symbol

PDCD1

UniProt

Q02242

Product Documents for Mouse PD‑1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse PD‑1 Antibody

For research use only

Citations for Mouse PD‑1 Antibody (11)

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Customer Reviews for Mouse PD‑1 Antibody (5)

4.6 out of 5
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  • Mouse PD-1 Antibody
    Name: Efstathios Stamatiades
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Mammary gland tissue
    Species: Mouse
    Verified Customer | Posted 07/09/2019
    Cryosections from PLP immersion fixed PyMT mammary gland tumors were stained for PD1 (1:50) for 4h @ RT. After washing, tissue was stained with Donkey anti-Goat alexa546 2ndary antibody for 1h at RT. CD3 in green, PD1 in red, nuclear staining (Hoechst) in blue
    Mouse PD‑1 Antibody AF1021
  • Mouse PD-1 Antibody
    Name: Ivan Luzardo
    Application: Western Blot
    Sample Tested: HCT-116 human colorectal carcinoma cell line
    Species: Human
    Verified Customer | Posted 03/28/2019
  • Mouse PD-1 Antibody
    Name: Anonymous
    Application: Functional Assay
    Sample Tested: mouse antibody
    Species: Mouse
    Verified Customer | Posted 03/07/2018
  • Mouse PD-1 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Human recombinant antibody
    Species: Mouse
    Verified Customer | Posted 09/29/2017
  • Mouse PD-1 Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Human T cell and Melanoma tissue
    Species: Human
    Verified Customer | Posted 06/15/2017

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