Mouse PD‑1 Antibody
Catalog # AF1021 | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
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Antibody Source
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Product Summary for Mouse PD‑1 Antibody
Immunogen
Leu25-Gln167
Accession # Q02242
Specificity
Clonality
Host
Isotype
Scientific Data Images for Mouse PD‑1 Antibody
Detection of Mouse PD‑1 by Western Blot.
Western blot shows lysates of EL-4 mouse lymphoblast cell line and CTLL-2 mouse cytotoxic T cell line (negative control). PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Mouse PD-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1021) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for PD-1 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Detection of Mouse PD‑1 by Western Blot.
Western blot shows lysates of 293T human embryonic kidney cell line mock transfected or transfected with mouse PD-1. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Mouse PD-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1021) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for PD-1 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.PD‑1 in Mouse Thymus.
PD‑1 was detected in perfusion fixed frozen sections of mouse thymus using 15 µg/mL Mouse PD‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1021) overnight at 4 °C. Tissue was stained (red) and counterstained (green). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.Applications for Mouse PD‑1 Antibody
CyTOF-ready
Flow Cytometry
Sample: Mouse splenocytes treated with PHA
Immunohistochemistry
Sample: Perfusion fixed frozen sections of mouse thymus and mouse spleen, and perfusion fixed paraffin-embedded sections of mouse thymus
Western Blot
Sample: EL‑4 mouse lymphoblast cell line and 293T human embryonic kidney cell line transfected with mouse PD-1
Reviewed Applications
Read 5 reviews rated 4.6 using AF1021 in the following applications:
Formulation, Preparation and Storage
Purification
Reconstitution
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PD-1
References
- Ishida, Y. et al. (1992) EMBO J. 11:3887.
- Sharpe, A.H. and G.J. Freeman (2002) Nat. Rev. Immunol. 2:116.
- Coyle, A. and J. Gutierrez-Ramos (2001) Nat. Immunol. 2:203.
- Nishimura, H. and T. Honjo (2001) Trends in Immunol. 22:265.
- Latchman Y. et al. (2001) Nature Immun. 2:261.
- Tamura, H. et al. (2001) Blood 97:1809.
Long Name
Alternate Names
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Gene Symbol
UniProt
Product Documents for Mouse PD‑1 Antibody
Product Specific Notices for Mouse PD‑1 Antibody
For research use only
Citations for Mouse PD‑1 Antibody (11)
Citations are publications that use Bio-Techne products. Selected citations for Mouse PD‑1 Antibody include:
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Species: Mouse
Sample Types: Whole Tissue
Applications: IHCT Kaya et al. (2022-10-24), CD8+ T cells induce interferon-responsive oligodendrocytes and microglia in white matter aging Nature Neuroscience, 2022-125(11):1446-1457.
PMID: 36280798 -
Species: Xenograft
Sample Types: Whole Tissue
Applications: IHCL Codarri De et al. (2022-09-28), PD-1-cis IL-2R agonism yields better effectors from stem-like CD8+ T cells Nature, 2022-0610(7930):161-172.
PMID: 36171284 -
Species: Mouse
Sample Types: Cell Lysates
Applications: Western BlotC Martins et al. (2022-07-21), Distinct antibody clones detect PD-1 checkpoint expression and block PD-L1 interactions on live murine melanoma cells Scientific Reports, 2022-012(1):12491.
PMID: 35864188 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCM Dudek et al. (2021-03-24), Auto-aggressive CXCR6+ CD8 T cells cause liver immune pathology in NASH Nature, 2021-00(0).
PMID: 33762736 -
Species: Mouse
Sample Types: Whole Cells,Whole Tissue
Applications: IHC,ICCT Lerbs et al. (2020-08-20), CD47 prevents the elimination of diseased fibroblasts in scleroderma JCI Insight, 2020-05(16).
PMID: 32814713 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCR Hoefflin et al. (2020-08-17), HIF-1alpha and HIF-2alpha differently regulate tumour development and inflammation of clear cell renal cell carcinoma in mice Nat Commun, 2020-011(1):4111.
PMID: 32807776 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-PF Ritzmann et al. (2019-07-17), IL-17C-mediated innate inflammation decreases the response to PD-1 blockade in a model of Kras-driven lung cancer Sci Rep, 2019-09(1):10353.
PMID: 31316109 -
Species: Human
Sample Types: Whole Tissue
Applications: IHCWJ Lee et al. (2019-03-14), Expression of lymphocyte-activating gene 3 and T-cell immunoreceptor with immunoglobulin and ITIM domains in cutaneous melanoma and their correlation with programmed cell death 1 expression in tumor-infiltrating lymphocytes J. Am. Acad. Dermatol., 2019-081(1):219-227.
PMID: 30880064 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCR Ruscher et al. (2017-05-22), CD8?? intraepithelial lymphocytes arise from two main thymic precursors Nat. Immunol., 2017-00(0).
PMID: 28530714 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-FrKasagi S et al. (2010-02-05), Anti-programmed cell death 1 antibody reduces CD4+PD-1+ T cells and relieves the lupus-like nephritis of NZB/W F1 mice. J. Immunol., 2010-0184(5):2337-47.
PMID: 20139271 -
Species: Mouse
Sample Types: Cell Lysates
Applications: Western BlotBishop KD et al. (2009-02-23), Depletion of the programmed death-1 receptor completely reverses established clonal anergy in CD4(+) T lymphocytes via an interleukin-2-dependent mechanism. Cell. Immunol., 2009-0256(1):86-91.
PMID: 19230866
There are no citations that match your criteria.
Customer Reviews for Mouse PD‑1 Antibody (5)
Customer Images
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Application: Immunocytochemistry/ImmunofluorescenceSample Tested: Mammary gland tissueSpecies: MouseVerified Customer | Posted 07/09/2019Cryosections from PLP immersion fixed PyMT mammary gland tumors were stained for PD1 (1:50) for 4h @ RT. After washing, tissue was stained with Donkey anti-Goat alexa546 2ndary antibody for 1h at RT. CD3 in green, PD1 in red, nuclear staining (Hoechst) in blue
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Application: Western BlotSample Tested: HCT-116 human colorectal carcinoma cell lineSpecies: HumanVerified Customer | Posted 03/28/2019
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Application: Functional AssaySample Tested: mouse antibodySpecies: MouseVerified Customer | Posted 03/07/2018
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Application: ELISASample Tested: Human recombinant antibodySpecies: MouseVerified Customer | Posted 09/29/2017
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Application: Flow CytometrySample Tested: Human T cell and Melanoma tissueSpecies: HumanVerified Customer | Posted 06/15/2017
There are no reviews that match your criteria.
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