Mouse Patched 1/PTCH Alexa Fluor® 594-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB11487T
Conjugate
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
Immunohistochemistry, Immunocytochemistry
Label
Alexa Fluor 594 (Excitation = 590 nm, Emission = 617 nm)
Antibody Source
Monoclonal Rat IgG2A Clone # 413218
Product Specifications
Immunogen
E. coli-derived recombinant mouse Patched 1/PTCH
Glu108-Asp422
Accession # Q61115
Glu108-Asp422
Accession # Q61115
Specificity
Detects mouse Patched 1/PTCH in direct ELISAs
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Applications
Application
Recommended Usage
Immunocytochemistry
Optimal dilution of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: Patched 1/PTCH
Patched is a 170‑175 kDa member of the patched family of proteins. It is found on multiple cells types and forms a constitutive, inactivating complex with Smoothened. Upon SHH binding to Patched, Smoothened is released and initiates downstream signaling. Mouse Patched is a 12 transmembrane, 1434 amino acid (aa) glycoprotein that contains two terminal cytoplasmic domains. Only one mRNA species for Patched has been reported in mice. However, it would appear likely that two or more splice forms exist, with variation occurring over the first 150 aa of the N-terminus. Over aa 108‑422, mouse Patched shares 97% and 99% aa sequence identity with human and rat Patched, respectively.
Alternate Names
BCNS, HPE7, mes, NBCCS, ptc1, PTCH1
Gene Symbol
PTCH1
UniProt
Additional Patched 1/PTCH Products
Product Specific Notices
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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