Mouse Mast Cell Protease-11/Prss34 Alexa Fluor® 532-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF2857X
Conjugate
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
Western Blot, Immunoprecipitation
Label
Alexa Fluor 532 (Excitation = 534 nm, Emission = 553 nm)
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Mast Cell Protease‑11/Prss34
Met20-Ser318
Accession # NP_848459
Met20-Ser318
Accession # NP_848459
Specificity
Detects mouse Mast Cell Protease‑11/Prss34 in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications
Application
Recommended Usage
Immunoprecipitation
Optimal dilution of this antibody should be experimentally determined.
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: Mast Cell Protease-11/Prss34
Mast Cell Protease-11 (MCP-11) is encoded by Prss34, one of 13 genes on mouse chromosome 17A3.3 that correspond to functional trypsin-like serine proteases (1). The deduced amino acid sequence of mouse MCP-11 consists of 318 residues with a signal peptide (residues 1 to 19), a pro region (residue 20 to 34), and a catalytic domain (35 to 318). The mRNA is preferentially expressed in spleen and bone marrow. The mouse MCP-11 (residues 20 to 318) was expressed in the NS0 cells with a foreign signal peptide. After being treated with thermolysin, the purified enzyme is active against a peptide substrate described in the Activity Assay Protocol. Apparently, the human gene corresponding to Prss34 encodes a protein that is not enzymatically active due to a mutation that leads to a premature translation termination codon.
Alternate Names
Mast Cell Protease11, MCP-11, MCPT11, Prss34
Entrez Gene IDs
328780 (Mouse)
Gene Symbol
PRSS34
UniProt
Additional Mast Cell Protease-11/Prss34 Products
Product Specific Notices
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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