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Key Product Details

Species Reactivity

Mouse

Applications

CyTOF-ready, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG1 Clone # 579511

Product Summary for Mouse MARCO Antibody

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse MARCO
Gln70-Ser518
Accession # Q60754

Specificity

Detects mouse MARCO in direct ELISAs.

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Scientific Data Images for Mouse MARCO Antibody

Detection of MARCO antibody in J774 Mouse Cell Line antibody by Flow Cytometry

Detection of MARCO in J774 Mouse Cell Line by Flow Cytometry

J774 mouse monocyte-macrophage cell line, resting (open histogram), or treated with 500 ng/ml LPS for 3 days (filled histogram) were stained with Rat Anti-Mouse MARCO Monoclonal Antibody (Catalog # MAB2956) followed by anti-Rat IgG APC-conjugated secondary antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse MARCO Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: J774 Mouse cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using MAB2956 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MARCO

Mouse MARCO is a type II transmembrane glycoprotein belonging to the class A scavenger receptor family. It is constitutively expressed in subsets of macrophages found in the marginal zone of the spleen, the peritoneum, and the medullary cord of lymph nodes. The extracellular domains of MARCO form a 220 kDa disulfide-linked homotrimer on the cell surface. MARCO binds both gram positive and gram negative bacteria, as well as oxidized low-density lipoprotein. The amino acid sequence of mouse MARCO extracellular domain is 69% identical to that of human MARCO.

Long Name

Macrophage Receptor with Collagenous Structure/Scavenger Receptor, Class A, Member 2

Alternate Names

SCARA2

Entrez Gene IDs

8685 (Human); 17167 (Mouse)

Gene Symbol

MARCO

UniProt

Product Documents for Mouse MARCO Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse MARCO Antibody

For research use only

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Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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