Mouse LIX Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAF433

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BAF433
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Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot, ELISA Detection (Matched Antibody Pair)

Cited:

ELISA Detection, ELISA Development, ELISA Development (Detection)

Label

Biotin

Antibody Source

Polyclonal Goat IgG

View all LIX Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse LIX
Val45-Ala118
Accession # P50228

Specificity

Detects mouse LIX in ELISAs and Western blots. In sandwich immunoassays, less than 0.05% cross-reactivity with recombinant human (rh) CXCL6/GCP-2, recombinant rat LIX, recombinant mouse (rm) CXCL13/BLC/BCA-1, rmCXCL11/I-TAC, rmCXCL9/MIG, rmCXCL4/PF4, and rmCXCL12/SDF-1 alpha is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse LIX Biotinylated Antibody

Mouse LIX ELISA Standard Curve

Mouse LIX ELISA Standard Curve

Recombinant Mouse LIX (Catalog # 433-MC) was serially diluted and captured by Rat Anti-Mouse LIX Monoclonal Antibody (Catalog # MAB433) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse LIX Antigen Affinity-purified Polyclonal Antibody (Catalog # AF433) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Mouse LIX Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse LIX (Catalog # 433-MC)

Mouse LIX Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)
Recommended Concentration: 0.1-0.4 µg/mL
Use in combination with these reagents:
  • Capture Reagent: Mouse LIX Antibody (Catalog # MAB433)
  • Standard: Recombinant Mouse LIX Protein (Catalog # 433-MC)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LIX

CXCL5 (LIX, Liposaccharide-Induced CXC chemokine; also GARG-8) is a secreted 8-9 kDa member of the Intercrine alpha (or CxC) family of chemokines. It is widely expressed, being produced by diverse cell types such as fibroblasts, thymic epithelium, platelets, vascular endothelium, hepatocytes, lung type II alveolar cells and ileal columnar epithelium. As a chemokine, LIX demonstrates chemokinetic properties. It induces the chemotaxis of neutrophils and endothelial cells, and also promotes TNF-alpha secretion from mast cells and macrophages. Notably, circulating LIX is not derived from fibroblasts, but platelets. This suggests that neutrophil homeostasis/chemotaxis is a function of local resident cell activation and LIX secretion, not generally circulating LIX. Mouse LIX is synthesized as a 132 amino acid (aa) precursor that contains a 40 aa signal sequence, a 78 aa mature region (aa 41-118), and a cleavable 14 aa C-terminus. The mature region possesses an ELR/GluLeuArg motif between aa 50-52, and an alpha-family characteristic CxC motif between aa 53-55. Although there are no known splice variants of mouse LIX, considerable proteolytic processing occurs at both the N- and C-termini over aa 41-132. This may reduce the MW in SDS-PAGE by as much as 3 kDa. The majority of LIX appears to start between aa 47-50, and this is positively correlated with bioactivity. Over aa 41-118, mouse LIX shares 73% aa sequence identity with rat LIX. Although not a strict ortholog, mouse LIX shares 63% aa sequence identity with human GCP-2.

Alternate Names

LIX

Entrez Gene IDs

20311 (Mouse); 60665 (Rat)

Gene Symbol

CXCL5

UniProt

P50228

Additional LIX Products

Product Documents for Mouse LIX Biotinylated Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Mouse LIX Biotinylated Antibody

For research use only

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