Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Neutralization, ELISA Capture, ELISA Development, ELISA Development (Capture), In vivo assay

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG_2B Clone # 61905

View all LIX Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse LIX
Val45-Ala118
Accession # P50228

Specificity

Detects mouse LIX in Western blots. In Western blots, no cross-reactivity with recombinant human CXCL1, 2 , 3 , 5 , 6 , 7 , 8 , 9, 10, 11, 12/SDF-1 alpha, 12/SDF-1 beta, 13, recombinant mouse CXCL1, 2, 6, 9, 10, 12/SDF-1 alpha, 13, recombinant rat CXCL1, 3/CINC-2 alpha, 3/CINC-2 beta, or recombinant porcine CXCL8 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG_2B

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Mouse LIX Antibody

Chemotaxis Induced by LIX and Neutralization by Mouse LIX Antibody.

Recombinant Mouse LIX (Catalog # 433-MC) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse LIX (0.2 µg/mL) is neutralized (green line) by increasing concen-trations of Rat Anti-Mouse LIX Monoclonal Antibody (Catalog # MAB433). The ND₅₀ is typically 0.5-2.5 µg/mL.

Mouse LIX ELISA Standard Curve

Recombinant Mouse LIX (Catalog # 433-MC) was serially diluted and captured by Rat Anti-Mouse LIX Monoclonal Antibody (Catalog # MAB433) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse LIX Antigen Affinity-purified Polyclonal Antibody (Catalog # AF433) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Mouse LIX Antibody

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Mouse LIX (Catalog # 433-MC)
under non-reducing conditions only

Neutralization

Measured by its ability to neutralize LIX-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND₅₀) is typically 0.5-2.5 µg/mL in the presence of 0.2 µg/mL Recombinant Mouse LIX.

Mouse LIX Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)

Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:

Detection Reagent: Mouse LIX Biotinylated Antibody (Catalog # BAF433)
Standard: Recombinant Mouse LIX Protein (Catalog # 433-MC)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using MAB433 in the following applications:

Western Blot (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LIX

LIX (Liposaccharide-Induced CXC chemokine; also GARG-8) is a secreted 8-9 kDa member of the Intercrine alpha (or CxC) family of chemokines. It is widely expressed, being produced by diverse cell types such as fibroblasts, thymic epithelium, platelets, vascular endothelium, hepatocytes, lung type II alveolar cells and ileal columnar epithelium. As a chemokine, LIX demonstrates chemokinetic properties. It induces the chemotaxis of neutrophils and endothelial cells, and also promotes TNF-alpha secretion from mast cells and macrophages. Notably, circulating LIX is not derived from fibroblasts, but platelets. This suggests that neutrophil homeostasis/chemotaxis is a function of local resident cell activation and LIX secretion, not generally circulating LIX. Mouse LIX is synthesized as a 132 amino acid (aa) precursor that contains a 40 aa signal sequence, a 78 aa mature region (aa 41-118), and a cleavable 14 aa C-terminus. The mature region possesses an ELR/GluLeuArg motif between aa 50-52, and an alpha-family characteristic CxC motif between aa 53-55. Although there are no known splice variants of mouse LIX, considerable proteolytic processing occurs at both the N- and C-termini over aa 41-132. This may reduce the MW in SDS-PAGE by as much as 3 kDa. The majority of LIX appears to start between aa 47-50, and this is positively correlated with bioactivity. Over aa 41-118, mouse LIX shares 73% aa sequence identity with rat LIX. Although not a strict ortholog, mouse LIX shares 63% aa sequence identity with human GCP-2.

Alternate Names

LIX

Entrez Gene IDs

20311 (Mouse); 60665 (Rat)

Gene Symbol

CXCL5

UniProt

P50228

Additional LIX Products

Product Documents for Mouse LIX Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse LIX Antibody

For research use only

Citations
Reviews

Mouse LIX Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB433

Key Product Details

Species Reactivity

Validated:

Cited:

Applications