Mouse Lipocalin-2/NGAL Antibody

Name: Mouse Lipocalin-2/NGAL Antibody
Brand: R&D Systems
SKU: AF1857
Rating: 5 (3 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # AF1857

Citations (56)

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Key Product Details

Species Reactivity:	Mouse
Immunogen:	Mouse myeloma cell line NS0-derived recombinant mouse Lipocalin-2/NGAL Gln21-Asn200 Accession # P11672
Applications:	Immunohistochemistry, Immunoprecipitation, Simple Western, Western Blot
Label:	Unconjugated
Source:	Polyclonal Goat IgG
Specificity:	Detects mouse Lipocalin-2/NGAL in direct ELISAs and Western blots.

View Full Technical Details for Mouse Lipocalin-2/NGAL Antibody

Applications for Mouse Lipocalin-2/NGAL Antibody

	Recommended Concentration	Sample
Western Blot	0.25 µg/mL	See below
Simple Western	5 µg/mL	See below
Immunohistochemistry	3-15 µg/mL	See below
Immunoprecipitation	25 µg/mL	Conditioned cell culture medium spiked with Recombinant Mouse Lipocalin‑2/NGAL (Catalog # 1857-LC), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Mouse Lipocalin-2/NGAL Antibody

	Western Blot Detection of Mouse Lipocalin‑2/NGAL by Western Blot. Western blot shows lysates of mouse uterus tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse Lipocalin-2/NGAL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1857) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for Lipocalin-2/NGAL at approximately 24 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
	Immunohistochemistry Lipocalin‑2/NGAL in Mouse Mammary Gland. Lipocalin-2/NGAL was detected in perfusion fixed frozen sections of mouse mammary gland using Goat Anti-Mouse Lipocalin-2/NGAL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1857) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to extracellular areas in ducts. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
	Simple Western Detection of Mouse Lipocalin‑2/NGAL by Simple Western^TM. Simple Western lane view shows lysates of mouse lung tissue, loaded at 0.2 mg/mL. A specific band was detected for Lipocalin-2/NGAL at approximately 37 kDa (as indicated) using 5 µg/mL of Goat Anti-Mouse Lipocalin-2/NGAL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1857) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: Lipocalin-2/NGAL

Mouse Lipocalin-2 was cloned from mouse kidney cells (1). Its very high level of expression at the post-stratum uterus gave it the name uterocalin (2). Lipocalin-2 has been implicated in a variety of processes including cell differentiation, tumorigenesis, and apoptosis (3- 5). Studies indicate that Lipocalin-2 binds a bacterial catecholate siderophore that is bound to a ferric ion, such as enterobactin, with a subnanomolar dissociation constant (K_d = 0.41 nM) (6). The bound ferric enterobactin complex breaks down slowly in a month into dihydroxybenzoyl serine and dihydroxybenzoic acid (DHBA). It also binds to a ferric DHBA complex with much less K_d values (7.9 nM) (6). Secretion of Lipocalin-2 in immune cells increases in response to stimulation of Toll-like receptor as an acute phase response to infection. As a result, it acts as a potent bacteriostatic reagent by sequestering iron (7). Moreover, Lipocalin-2 can alter the invasive and metastatic behavior of Ras-transformed breast cancer cells in vitro and in vivo by reversing the epithelial to mesenchymal transition inducing activity of Ras, through restoration of E-cadherin expression, via effects on the Ras-MAPK signaling pathway (8).

Item	Value
References	Hraba-Renevey, S. et al. (1989) Oncogene. 4:601. Liu, Q. et al. (1993) Mol Reprod Dev. 46:507. Kjeldsen L, et al. (2000) Biochim Biophys Acta. 1482:272. Devireddy, L.R. et al. (2001) Science 293:829. Yang, M.B. et al. (2002) Mol. Cell. 10:1045. Goetz, D.H. et al. (2002) Mol. Cell 10:1033. Flo, T.H. et al. (2004) Nature 432:917. Hanai, J. et al. (2005) J. Biol. Chem. 280:13641.
Long Name	Neutrophil Gelatinase-associated Lipocalin
Entrez Gene IDs	3934 (Human); 16819 (Mouse); 170496 (Rat); 102137149 (Cynomolgus Monkey)
Alternate Names	24p3, 25 kDa alpha-2-microglobulin-related subunit of MMP-9, HNL, LCN2, Lipocalin-2, Lipocalin2, MSFI, NGAL, NGALlipocalin-2, Oncogene 24p3, Siderocalin, Uterocalin, lipocalin 2, lipocalin 2 (oncogene 24p3), migration-stimulating factor inhibitor, neutrophil gelatinase-associated lipocalin, p25

Citations for Mouse Lipocalin-2/NGAL Antibody (56)

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Citations are publications that use Bio-Techne products. Selected citations for Mouse Lipocalin-2/NGAL Antibody include:

Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot

TH Shen et al. (2022), Snapshots of nascent RNA reveal cell- and stimulus- specific responses to acute kidney injury JCI Insight, 0(0).
PMID: 35230973
Species: Mouse
Sample Types: Whole Cells
Applications: ICC

T Wan et al. (2022), Astrocytic phagocytosis contributes to demyelination after focal cortical ischemia in mice Nature Communications, 13(1):1134.
PMID: 35241660
Species: Mouse
Sample Types: Tissue Lysates
Applications: Western Blot

T Eckes et al. (2022), Sphingosine 1-Phosphate Receptor 5 (S1P5) Knockout Ameliorates Adenine-Induced Nephropathy International Journal of Molecular Sciences, 23(7).
PMID: 35409312
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

X Xiang et al. (2022), Role of lipocalin-2 in surgery-induced cognitive decline in mice: a signal from neuron to microglia Journal of Neuroinflammation, 19(1):92.
PMID: 35413913
Species: Mouse
Sample Types: Cell Lysates
Applications: IHC

M Silva Oliv et al. (2022), Myelin repair is fostered by the corticosteroid medrysone specifically acting on astroglial subpopulations EBioMedicine, 83(0):104204.
PMID: 35952494
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot

HJ Shin et al. (2022), Lipocalin-2 Deficiency Reduces Hepatic and Hippocampal Triggering Receptor Expressed on Myeloid Cells-2 Expressions in High-Fat Diet/Streptozotocin-Induced Diabetic Mice Brain sciences, 12(7).
PMID: 35884685
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

R Greite et al. (2022), Different Acute Kidney Injury Patterns after Renal Ischemia Reperfusion Injury and Extracorporeal Membrane Oxygenation in Mice International Journal of Molecular Sciences, 23(19).
PMID: 36232304
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

C Zhang et al. (2022), Histone methyltransferase MLL1 drives renal tubular cell apoptosis by p53-dependent repression of E-cadherin during cisplatin-induced acute kidney injury Cell Death Disease, 2022;13(9):770.
PMID: 36068197
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot

JR Sheldon et al. (2022), Lipocalin-2 is an essential component of the innate immune response to Acinetobacter baumannii infection PloS Pathogens, 18(9):e1010809.
PMID: 36054235
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

B Olson et al. (2021), Lipocalin 2 mediates appetite suppression during pancreatic cancer cachexia Nature Communications, 12(1):2057.
PMID: 33824339
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

B Olson et al. (2021), Chronic cerebral lipocalin 2 exposure elicits hippocampal neuronal dysfunction and cognitive impairment Brain, Behavior, and Immunity, 0(0).
PMID: 34245812
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot

N Helmrich et al. (2021), Pharmacological Antagonization of Cannabinoid Receptor 1 Improves Cholestasis in Abcb4-/- Mice Cellular and Molecular Gastroenterology and Hepatology, 0(0).
PMID: 34954190
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

F Yao et al. (2021), A targetable LIFR-NF-kappaB-LCN2 axis controls liver tumorigenesis and vulnerability to ferroptosis Nature Communications, 12(1):7333.
PMID: 34921145
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot

N Ma et al. (2021), Farrerol Ameliorated Cisplatin-Induced Chronic Kidney Disease Through Mitophagy Induction via Nrf2/PINK1 Pathway Frontiers in Pharmacology, 12(0):768700.
PMID: 34858188
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot

S Talley et al. (2021), DSS-induced inflammation in the colon drives a proinflammatory signature in the brain that is ameliorated by prophylactic treatment with the S100A9 inhibitor paquinimod Journal of Neuroinflammation, 18(1):263.
PMID: 34758843
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

H Ahn et al. (2021), NLRP3 Triggers Attenuate Lipocalin-2 Expression Independent with Inflammasome Activation Cells, 10(7).
PMID: 34359830
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

EM Buhl et al. (2020), Dysregulated mesenchymal PDGFR-&beta drives kidney fibrosis EMBO Mol Med, 0(0):e11021.
PMID: 31943786
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

A Mondal et al. (2020), Lipocalin 2 induces neuroinflammation and blood-brain barrier dysfunction through liver-brain axis in murine model of nonalcoholic steatohepatitis J Neuroinflammation, 17(1):201.
PMID: 32622362
Species: Mouse
Sample Types: Protein
Applications: Western Blot

E Staurenghi et al. (2020), Oxysterols present in Alzheimer's disease brain induce synaptotoxicity by activating astrocytes: A major role for lipocalin-2 Redox Biology, 39(0):101837.
PMID: 33360775
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

K Noh et al. (2019), Negr1 controls adult hippocampal neurogenesis and affective behaviors Mol. Psychiatry, 0(0).
PMID: 30651602
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

KJ Meade et al. (2019), Secretomes from metastatic breast cancer cells, enriched for a prognostically unfavorable LCN2 axis, induce anti-inflammatory MSC actions and a tumor-supportive premetastatic lung Oncotarget, 10(32):3027-3039.
PMID: 31105883
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot

K Chella Kri et al. (2019), Sex-specific metabolic functions of adipose Lipocalin-2 Mol Metab, 30(0):30-47.
PMID: 31767179
Species: Human
Sample Types: Cell Lysate
Applications: IP

L Yammine et al. (2019), Lipocalin-2 Regulates Epidermal Growth Factor Receptor Intracellular Trafficking Cell Rep, 29(7):2067-2077.e6.
PMID: 31722218
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot

S Ueno et al. (2018), Early Gene Expression Profile in Retinal Ganglion Cell Layer After Optic Nerve Crush in Mice Invest. Ophthalmol. Vis. Sci., 59(1):370-380.
PMID: 29346801
Species: Mouse
Sample Types: Urine
Applications: Western Blot

H Langhorst et al. (2018), The IgCAM CLMP is required for intestinal and ureteral smooth muscle contraction by regulating Connexin43 and 45 expression in mice Dis Model Mech, 0(0).
PMID: 29361518
Species: Mouse
Sample Types: Urine
Applications: Western Blot

TA Yakulov et al. (2018), CXCL12 and MYC control energy metabolism to support adaptive responses after kidney injury Nat Commun, 9(1):3660.
PMID: 30202007
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

Y Kim et al. (2017), Elevated urinary CRELD2 is associated with endoplasmic reticulum stress-mediated kidney disease JCI Insight, 2(23).
PMID: 29212948
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot

M Trudu et al. (2017), Early involvement of cellular stress and inflammatory signals in the pathogenesis of tubulointerstitial kidney disease due to UMOD mutations Sci Rep, 7(1):7383.
PMID: 28785050
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

A Eskilsson et al. (2017), Immune-induced fever is dependent on local but not generalized prostaglandin E2 synthesis in the brain J. Neurosci., 0(0).
PMID: 28438967
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot

I Mosialou et al. (2017), MC4R-dependent suppression of appetite by bone-derived lipocalin 2 Nature, 543(7645):385-390.
PMID: 28273060
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot

(2016), Keap1 hypomorphism protects against ischemic and obstructive kidney disease Sci Rep, 6(0):36185.
PMID: 27804998
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot

(2016), Beneficial Effects of Sodium Phenylbutyrate Administration During Infection with Salmonella enterica serovar Typhimurium Infect Immun, 0(0).
PMID: 27382022
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot

GJ Song et al. (2016), A novel role for protein tyrosine phosphatase 1B as a positive regulator of neuroinflammation J Neuroinflammation, 13(1):86.
PMID: 27095436
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot

(2016), Influenza Virus Affects Intestinal Microbiota and Secondary Salmonella Infection in the Gut through Type I Interferons PLoS Pathog, 12(5):e1005572.
PMID: 27149619
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr

(2016), Cyclooxygenase Isoform Exchange Blocks Brain-Mediated Inflammatory Symptoms PLoS ONE, 11(11):e0166153.
PMID: 27861574
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot

Drew B et al. (2015), Estrogen receptor (ER)alpha-regulated lipocalin 2 expression in adipose tissue links obesity with breast cancer progression. J Biol Chem, 290(9):5566-81.
PMID: 25468909
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

Weng Y et al. (2015), Identification of lipocalin-2 as a PKCdelta phosphorylation substrate in neutrophils. J Biomed Sci, 22(0):21.
PMID: 25890235
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Rucci N et al. (2015), Lipocalin 2: a new mechanoresponding gene regulating bone homeostasis. J Bone Miner Res, 30(2):357-68.
PMID: 25112732
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot

Behnsen J et al. (2014), The cytokine IL-22 promotes pathogen colonization by suppressing related commensal bacteria. Immunity, 40(2):262-73.
PMID: 24508234
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot

Nocon A et al. (2014), The bacteriostatic protein lipocalin 2 is induced in the central nervous system of mice with west Nile virus encephalitis. J Virol, 88(1):679-89.
PMID: 24173226
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

Glenthoj A et al. (2014), Human alpha-defensin expression is not dependent on CCAAT/enhancer binding protein-epsilon in a murine model. PLoS ONE, 9(3):e92471.
PMID: 24658030
Species: Mouse
Sample Types: Urine
Applications: Western Blot

Paragas N et al. (2014), alpha-Intercalated cells defend the urinary system from bacterial infection. J Clin Invest, 124(7):2963-76.
PMID: 24937428
Miao Q et al. (2014), Tcf3 promotes cell migration and wound repair through regulation of lipocalin 2. Nat Commun, 5(0):4088.
PMID: 24909826
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot

Zhang Y et al. (2014), Lipocalin 2 regulates brown fat activation via a nonadrenergic activation mechanism. J Biol Chem, 289(32):22063-77.
PMID: 24917675
Species: Mouse
Sample Types: Urine
Applications: Western Blot

Grahammer F et al. (2014), mTORC1 maintains renal tubular homeostasis and is essential in response to ischemic stress. Proc Natl Acad Sci U S A, 111(27):E2817-26.
PMID: 24958889
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

Guo H et al. (2014), Lipocalin 2 is a regulator of macrophage polarization and NF-kappaB/STAT3 pathway activation. Mol Endocrinol, 28(10):1616-28.
PMID: 25127375
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

Tasso et al. (2013), In vivo implanted bone marrow-derived mesenchymal stem cells trigger a cascade of cellular events leading to the formation of an ectopic bone regenerative niche. Stem Cells Dev, 22(24):3178-91.
PMID: 23924051
Species: Mouse
Sample Types: Whole Cells
Applications: ICC

Malhotra D et al. (2012), Transcriptional profiling of stroma from inflamed and resting lymph nodes defines immunological hallmarks. Nat. Immunol., 13(5):499-510.
PMID: 22466668
Species: Mouse
Sample Types: BALF
Applications: Western Blot

Louten J et al. (2012), Biomarkers of disease and treatment in murine and cynomolgus models of chronic asthma. Biomark Insights, 7(0):87-104.
PMID: 22837640
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

Cramer EP et al. (2012), No effect of NGAL/lipocalin-2 on aggressiveness of cancer in the MMTV-PyMT/FVB/N mouse model for breast cancer. PLoS ONE, 7(6):e39646.
PMID: 22737251
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot

Ip JP et al. (2011), Lipocalin 2 in the central nervous system host response to systemic lipopolysaccharide administration. J Neuroinflammation, 8(0):124.
PMID: 21943033
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

Mahadevan NR et al. (2011), ER stress drives Lipocalin 2 upregulation in prostate cancer cells in an NF-kappaB-dependent manner. BMC Cancer, 11(0):229.
PMID: 21649922
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

Lee S et al. (2009), Lipocalin-2 is an autocrine mediator of reactive astrocytosis. J. Neurosci., 29(1):234-49.
PMID: 19129400
Species: Mouse
Sample Types: Urine
Applications: Western Blot

Kuwabara T et al. (2009), Urinary neutrophil gelatinase-associated lipocalin levels reflect damage to glomeruli, proximal tubules, and distal nephrons. Kidney Int., 75(3):285-94.
PMID: 19148153
Species: Mouse
Sample Types: Plasma
Applications: Western Blot

Yan QW et al. (2007), The adipokine lipocalin 2 is regulated by obesity and promotes insulin resistance. Diabetes, 56(10):2533-40.
PMID: 17639021
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr

Andre E et al. (2006), Inhalation of ultrafine carbon particles triggers biphasic pro-inflammatory response in the mouse lung. Eur. Respir. J., 28(2):275-85.
PMID: 16641123

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Customer Reviews for Mouse Lipocalin-2/NGAL Antibody (3)

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Mouse Lipocalin-2/NGAL Antibody

Name: Anonymous

Application: ICC/IF

Sample Tested: Brain tissue

Species: Mouse

Verified Customer | Posted 11/06/2020
Mouse Lipocalin-2/NGAL Antibody

Name: Anonymous

Application: ICC/IF

Sample Tested: Differentiated osteoblasts

Species: Mouse

Verified Customer | Posted 02/16/2018

incubated O/N with AF-1857 (1:500) at 4oC.
Mouse Lipocalin-2/NGAL Antibody

Name: Anonymous

Application: WB

Sample Tested: Kidney tissue

Species: Mouse

Verified Customer | Posted 01/06/2017

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Mouse Lipocalin-2/NGAL Antibody

R&D Systems, Inc. a Bio-Techne Brand

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Applications for Mouse Lipocalin-2/NGAL Antibody

Scientific Data Examples for Mouse Lipocalin-2/NGAL Antibody

Western Blot

Immunohistochemistry

Simple Western

Formulation, Preparation and Storage

Reconstitution Calculator

Background: Lipocalin-2/NGAL

Citations for Mouse Lipocalin-2/NGAL Antibody (56)

Customer Reviews for Mouse Lipocalin-2/NGAL Antibody (3)

Customer Images

Mouse Lipocalin-2/NGAL Antibody

Mouse Lipocalin-2/NGAL Antibody

Mouse Lipocalin-2/NGAL Antibody

Product Documents for Mouse Lipocalin-2/NGAL Antibody

Product Datasheets

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