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Mouse Lipocalin-2/NGAL Antibody

Bio-Techne includes R&D Systems | Catalog # AF1857

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AF1857
AF1857-SP

Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry, Immunoprecipitation, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Product Summary for Mouse Lipocalin-2/NGAL Antibody

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Lipocalin-2/NGAL
Gln21-Asn200
Accession # P11672

Specificity

Detects mouse Lipocalin-2/NGAL in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse Lipocalin-2/NGAL Antibody

Detection of Mouse Lipocalin-2/NGAL antibody by Western Blot.

Detection of Mouse Lipocalin‑2/NGAL by Western Blot.

Western blot shows lysates of mouse uterus tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse Lipocalin-2/NGAL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1857) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for Lipocalin-2/NGAL at approximately 24 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Lipocalin-2/NGAL antibody in Mouse Mammary Gland by Immunohistochemistry (IHC-Fr).

Lipocalin‑2/NGAL in Mouse Mammary Gland.

Lipocalin-2/NGAL was detected in perfusion fixed frozen sections of mouse mammary gland using Goat Anti-Mouse Lipocalin-2/NGAL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1857) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to extracellular areas in ducts. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Detection of Mouse Lipocalin-2/NGAL antibody by Simple WesternTM.

Detection of Mouse Lipocalin-2/NGAL by Simple WesternTM.

Simple Western lane view shows lysates of mouse lung tissue, loaded at 0.2 mg/mL. A specific band was detected for Lipocalin-2/NGAL at approximately 37 kDa (as indicated) using 5 µg/mL of Goat Anti-Mouse Lipocalin-2/NGAL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1857) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Mouse Lipocalin-2/NGAL Antibody

Application
Recommended Usage

Immunohistochemistry

3-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse mammary gland

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Mouse Lipocalin-2/NGAL (Catalog # 1857-LC), see our available Western blot detection antibodies

Simple Western

5 µg/mL
Sample: Mouse lung tissue

Western Blot

0.25 µg/mL
Sample: Mouse uterus tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 5 reviews rated 5 using AF1857 in the following applications:

Published Applications

Read 103 publications using AF1857 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Lipocalin-2/NGAL

Mouse Lipocalin-2 was cloned from mouse kidney cells (1). Its very high level of expression at the post-stratum uterus gave it the name uterocalin (2). Lipocalin-2 has been implicated in a variety of processes including cell differentiation, tumorigenesis, and apoptosis (3- 5). Studies indicate that Lipocalin-2 binds a bacterial catecholate siderophore that is bound to a ferric ion, such as enterobactin, with a subnanomolar dissociation constant (Kd = 0.41 nM) (6). The bound ferric enterobactin complex breaks down slowly in a month into dihydroxybenzoyl serine and dihydroxybenzoic acid (DHBA). It also binds to a ferric DHBA complex with much less Kd values (7.9 nM) (6). Secretion of Lipocalin-2 in immune cells increases in response to stimulation of Toll-like receptor as an acute phase response to infection. As a result, it acts as a potent bacteriostatic reagent by sequestering iron (7). Moreover, Lipocalin-2 can alter the invasive and metastatic behavior of Ras-transformed breast cancer cells in vitro and in vivo by reversing the epithelial to mesenchymal transition inducing activity of Ras, through restoration of E-cadherin expression, via effects on the Ras-MAPK signaling pathway (8).

References

  1. Hraba-Renevey, S. et al. (1989) Oncogene. 4:601. 
  2. Liu, Q. et al. (1993) Mol Reprod Dev. 46:507. 
  3. Kjeldsen L, et al. (2000) Biochim Biophys Acta. 1482:272.
  4. Devireddy, L.R. et al. (2001) Science 293:829.
  5. Yang, M.B. et al. (2002) Mol. Cell. 10:1045.
  6. Goetz, D.H. et al. (2002) Mol. Cell 10:1033.
  7. Flo, T.H. et al. (2004) Nature 432:917.
  8. Hanai, J. et al. (2005) J. Biol. Chem. 280:13641.

Long Name

Neutrophil Gelatinase-associated Lipocalin

Alternate Names

24p3, LCN2, Lipocalin2, MSFI, NGAL, Oncogene 24p3, p25, Siderocalin, Uterocalin

Entrez Gene IDs

3934 (Human); 16819 (Mouse); 170496 (Rat); 102137149 (Cynomolgus Monkey)

Gene Symbol

LCN2

UniProt

Additional Lipocalin-2/NGAL Products

Product Documents for Mouse Lipocalin-2/NGAL Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse Lipocalin-2/NGAL Antibody

For research use only

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