D3 mouse embryonic stem cell line was stained with Rat Anti-Mouse LIF Ra APC-conjugated Monoclonal Antibody (Catalog # FAB5990A, filled histogram) or isotype control antibody (Catalog # IC005A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse LIFR alpha APC-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: D3 mouse embryonic stem cell line

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: LIFR alpha

Leukemia Inhibitory Factor Receptor alpha (LIF R alpha), also known as LIFR beta (due to its participation in signal transduction) and CD118, is a 190 kDa type I transmembrane protein in the Interleukin-6 receptor family. Members of this family mediate the biological effects of Cardiotrophin-1, CLC, CNTF, IL-6, IL-11, IL-27, and Oncostatin M (1). Mature mouse LIF R alpha consists of a 785 amino acid (aa) extracellular domain (ECD) with two cytokine receptor homology domains, one WSxWS motif, and three fibronectin type III repeats, followed by a 25 aa transmembrane segment and a 239 aa cytoplasmic domain (2, 3). Within the ECD, mouse LIF R alpha shares 73% and 90% aa sequence identity with human and rat LIF R alpha, respectively. Alternative splicing generates a 90 kDa soluble form of the mouse LIF R alpha ECD (4). LIF R alpha binds the pleiotropic cytokine LIF with low affinity, and the soluble isoform retains LIF-binding activity (5). Binding affinity is increased by the ligand-induced association of LIF R alpha with the signal transducing subunit gp130 (6, 7). The LIF R alpha/gp130 receptor complex also transduces Oncostatin M signals, although LIF R alpha alone does not interact with Oncostatin M (6). gp130 associates with different ligand-specific receptors to form signaling receptor complexes for the other IL-6 family ligands (1). The CNTF receptor is a ternary complex that contains CNTF R alpha and gp130 as well as LIF R alpha (8, 9). LIF R alpha is widely expressed, and LIF induces the proliferation, differentiation, and activation of cells in many tissues (10, 11). In particular, LIF R alpha plays an important role in several aspects of early pregnancy such as blastocyst implantation in the uterus (4, 12-14).

References

Muller-Newen, G. (2003) Science STKE 2003:pe40.
Gearing, D.P. et al. (1991) EMBO J. 10:2839.
Tomida, M. et al. (1994) J. Biochem. 115:557.
Owczarek, C.M. et al. (1996) J. Biol. Chem. 271:5495.
Layton, M.J. et al. (1992) Proc. Natl. Acad. Sci. USA 89:8616.
Gearing, D.P. et al. (1992) Science 255:1434.
Giese, B. et al. (2005) J. Cell Sci. 118:5129.
Ip, N.Y. et al. (1992) Cell 69:1121.
Davis, S. et al. (1993) Science 260:1805.
Metcalf, D (2003) Stem Cells 21:5.
Kubota, Y. et al. (2008) J. Clin. Invest. 118:2393.
Paiva, P. et al. (2009) Cytokine Growth Factor Rev. 20:319.
Stewart, C.L. et al. (1992) Nature 359:76.
Cheng, J.-G. et al. (2001) Proc. Natl. Acad. Sci. USA 98:8680.

Long Name

Leukemia Inhibitory Factor Receptor alpha

Alternate Names

CD118, LIF R alpha, LIF R beta, LIFR, LIFRa

Entrez Gene IDs

3977 (Human); 16880 (Mouse); 81680 (Rat); 102131906 (Cynomolgus Monkey)

Gene Symbol

LIFR

UniProt

P42703

Additional LIFR alpha Products

Product Documents for Mouse LIFR alpha APC-conjugated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse LIFR alpha APC-conjugated Antibody

For research use only

Citations
Reviews

Mouse LIFR alpha APC-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB5990A

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all LIFR alpha Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Mouse LIFR alpha APC-conjugated Antibody

Detection of LIF R alpha in D3 Mouse Cell Line by Flow Cytometry.

Applications for Mouse LIFR alpha APC-conjugated Antibody

Flow Cytometry

Spectra Viewer

Plan Your Experiments

Formulation, Preparation, and Storage

Purification

Formulation

Shipping

Stability & Storage

Background: LIFR alpha

References

Long Name

Alternate Names

Entrez Gene IDs

Gene Symbol

UniProt

Additional LIFR alpha Products

Product Documents for Mouse LIFR alpha APC-conjugated Antibody

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for Mouse LIFR alpha APC-conjugated Antibody