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Mouse Lefty-2 Antibody

Catalog # AF7648 | R&D Systems, Inc. a Bio-Techne Brand
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Key Product Details

Species Reactivity



Immunocytochemistry, Western Blot



Antibody Source

Polyclonal Sheep IgG

Product Summary for Mouse Lefty-2 Antibody


Chinese hamster ovary cell line CHO-derived recombinant mouse Lefty-2
Accession # P57785


Detects mouse Lefty-2 in direct ELISAs and Western blots. In direct ELISAs, approximately 65% cross-reactivity with recombinant mouse Lefty-1 is observed, and approximately 20% cross-reactivity with recombinant human Lefty-2 is observed.







Scientific Data Images for Mouse Lefty-2 Antibody

Detection of Mouse Lefty-2 antibody by Western Blot.

Detection of Mouse Lefty-2 by Western Blot.

Western blot shows lysates of P19 mouse embryonal carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Mouse Lefty-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7648) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Lefty-2 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Lefty-2 antibody in D3 Mouse Embryonic Stem Cells by Immunocytochemistry (ICC).

Lefty-2 in D3 Mouse Embryonic Stem Cells.

Lefty-2 was detected in immersion fixed D3 mouse embryonic stem cells untreated (lower panel) or stimulated (upper panel) with recombinant human/mouse/rat Activin A (Catalog # 338-AC) using Sheep Anti-Mouse Lefty-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7648) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to the cytoplasm of Activin A-stimulated cells. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Mouse Lefty-2 Antibody

Recommended Usage


5-15 µg/mL
Sample: Immersion fixed D3 mouse embryonic stem cells untreated or stimulated with recombinant human/mouse/rat Activin A (Catalog # 338-AC)

Western Blot

2 µg/mL
Sample: P19 mouse embryonal carcinoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation and Storage


Antigen Affinity-purified


Sterile PBS to a final concentration of 0.2 mg/mL.

Reconstitution Buffer Available:
Size / Price


Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Lefty-2

Lefty-2 (Left-right determination factor 2; also Lefty-B [in human]) is a atypical member of the TGF-beta family of proteins. It is expressed during early embryogenesis in both the primitive streak and left-side lateral plate mesoderm. In the adult, Lefty-2 appears in oviduct epithelium. Lefty-2 acts in a manner reminiscent of that for Chordin and Noggin, and it is assumed that Lefty-2 is an antagonist of BMP activity. Notably, Lefty-2 and Nodal are likely under the control of Lefty-1, and thus all three molecules would appear to contribute to the creation of a left side-type body plan. Mouse Lefty-2 is synthesized as a 368 amino acid (aa) preproprecursor. It contains a 21 aa signal sequence, plus a 347 aa, 41-42 kDa bioactive proprecursor that may undergo proteolytic processing at one of two downstream cleavage sites. If cleavage occurs after Arg77, the resulting 33-34 kDa mature form (aa 78-368) is biologically inactive; if cleavage occurs after Arg135, the resulting 27-28 kDa mature form (aa 136-368) is biologically active. Lefty-2 is not a covalent homodimer and has been suggested to act as a monomer. Over aa 78-368, mouse Lefty-2 shares 94%, 83% and 95% aa sequence identity with rat Lefty-2, human Lefty-B and mouse Lefty-1, respectively.

Alternate Names

Ebaf, TGF-beta 4, TGFB4

Entrez Gene IDs

7044 (Human); 320202 (Mouse); 289316 (Rat)

Gene Symbol



Product Documents for Mouse Lefty-2 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse Lefty-2 Antibody

For research use only

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