Integrin alpha10 beta1 is one of twelve integrin family adhesion receptors that share the beta1 subunit (1‑3). The non‑covalent heterodimer of 160 kDa alpha11 and 130 kDa beta1/CD29 type I transmembrane glycoprotein subunits is expressed mainly on chondrocytes within cartilage, but also in fibrous connective tissues such as heart valves and ligaments (3, 4). The alpha10 extracellular domain (ECD) contains an I (inserted) domain which includes the ligand binding site (2, 3, 5). The beta1 ECD contains a vWFA domain, which participates in binding. Each subunit then has a transmembrane sequence and a short cytoplasmic tail. The dimer is folded when it is least active. Divalent cations and intracellular (inside‑out) signaling convert it to its most active, extended and open conformation (1, 2). The 1100 amino acid (aa) mouse alpha10 extracellular domain (ECD) shares 96% aa sequence identity with rat and 88‑89% with human, rabbit, porcine, canine and bovine alpha10, while the 708 aa mouse beta1 ECD shares 98% aa identity with rat and 93‑94% with human, bovine, porcine, ovine, canine and feline beta1. A potential mouse alpha10 splice variant diverges at aa 1039 and is terminated prematurely. If translated, this variant would result in a secreted protein (6). I domain‑containing beta1 integrins alpha1 beta1, alpha2 beta1, alpha10 beta1 and alpha11 beta1 all bind collagens; all but alpha11 beta1 also bind laminins (5, 7, 8). During cartilage differentiation, alpha10 beta1 is thought to be the main integrin binding type II and IX cartilage collagens (3‑5, 7‑10). However, deletion of mouse alpha10 causes a mild phenotype including slightly shortened bones and narrowed hypertrophic zones, indicating that another collagen‑binding integrin, likely alpha2 beta1, may compensate for alpha10 beta1 functions (11). Migration of melanoma cells has been noted to correlate with alpha10 beta1 expression (12).

References

Takada, Y. et al. (2007) Genome Biol. 8:215.
Luo, B-H. et al. (2007) Annu. Rev. Immunol. 25:619.
Camper, L. et al. (1998) J. Biol. Chem. 273:20383.
Camper, L. et al. (2001) Cell Tiss. Res. 306:107.
Tulla, M. et al. (2001) J. Biol. Chem. 276:48206.
Bengtsson, T. et al. (2001) Matrix Biol. 20:565.
McCall-Culbreath, K.D. and M.M. Zutter (2008) Curr. Drug Targets 9:139.
Popova, S.N. et al. (2007) Acta Physiol. 190:179.
Varas, L. et al. (2007) Stem Cells Dev. 16:965.
Gigout, A. et al. (2008) J. Biol. Chem. 283:31522.
Bengtsson, T. et al. (2004) J. Cell Sci. 118:939.
Wenke, A.K. et al. (2007) Cell Oncol. 29:373.

Alternate Names

ITGA10, PRO827

Entrez Gene IDs

8515 (Human); 213119 (Mouse); 310683 (Rat)

Gene Symbol

ITGA10

UniProt

NP_001289400.1 (Integrin alpha10) and P09055 (Integrin beta1)

Additional Integrin alpha 10 Products

Product Documents

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

Citations
Reviews

Spectra Viewer
Flow Cytometry

Mouse Integrin alpha10 Alexa Fluor™ Plus 488-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB9757AFP488

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all Integrin alpha 10 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Applications

CyTOF-ready

Flow Cytometry

Formulation, Preparation, and Storage

Formulation

Shipping

Stability & Storage

Background: Integrin alpha 10