Mouse IL-6R alpha Antibody

Name: Mouse IL-6R alpha Antibody
Brand: R&D Systems
SKU: MAB1830

R&D Systems, Inc. a Bio-Techne Brand

Catalog # MAB1830

Citations (3)

Product Datasheet / COA / SDS

Catalog #

Availability

Size / Price

Qty

Setting the standard in quality research reagents

Key Product Details

Species Reactivity:	Mouse
Immunogen:	Mouse myeloma cell line NS0-derived recombinant mouse IL-6 R alpha Leu20-Glu357 Accession # P22272
Applications:	CyTOF-ready, Flow Cytometry, Immunocytochemistry/Immunofluorescence
Label:	Unconjugated
Source:	Monoclonal Rat IgG_2A Clone # 255821
Specificity:	Detects mouse IL‑6 R alpha in direct ELISAs. In direct ELISAs, no cross‑reactivity with recombinant human IL-6 R alpha is observed.

View Full Technical Details for Mouse IL-6R alpha Antibody

Complete Your Research

Applications for Mouse IL-6R alpha Antibody

	Recommended Concentration	Sample
Flow Cytometry	2.5 µg/10⁶ cells	Mouse CD3⁺ splenocytes
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry	8-25 µg/mL	Immersion fixed mouse splenocytes

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: IL-6R alpha

Interleukin 6 (IL-6) is a multifunctional cytokine that exerts its activities by binding to a high-affinity receptor complex consisting of two membrane glycoproteins: an 80 kDa ligand binding subunit (IL-6 R alpha /CD126) and a 130 kDa nonligand-binding signal-transducing subunit (gp130/CD130) (1‑4). Mature mouse IL-6 R alpha is a type I transmembrane protein of 460 amino acids (aa) that contains a 345 aa extracellular ligand binding domain, a 21 aa transmembrane region, and a 75 aa cytoplasmic segment (2). The extracellular segment contains an Ig-like and a fibronectin-type III domain, plus a membrane proximal WSxWS motif. In their extracellular regions, mouse IL‑6 R alpha shares 89%, 51% and 50% aa identity with rat, human and porcine IL‑6 R alpha, respectively. Unlike gp130 that is expressed ubiquitously, the cellular distribution of IL-6 R alpha is predominantly limited to hepatocytes and leukocyte subpopulations such as monocytes, neutrophils, T and B cells. Soluble IL-6R alpha has been found in various body fluids (5). Two soluble receptor isoforms that arise either from proteolytic cleavage of the membrane-bound IL‑6 R alpha, or by alternative mRNA splicing (reported only in human) have been described (6, 7). Soluble IL-6 R alpha binds IL-6 with an affinity similar to that of the membrane-bound IL-6 R alpha. More importantly, the soluble IL-6 R alpha /IL-6 complex is capable of interacting with the membrane-bound gp130 to activate cells that lack an integral membrane IL-6 R alpha. It has been documented that elevated soluble IL-6 R is associated with numerous diseases including arthritic lesions, multiple myeloma and Crohn’s disease (6, 7).

Item	Value
References	Yamasaki, K. et al. (1988) Science 241:825. Sugita, T. et al. (1990) J. Exp. Med. 171:2001. Hibi, M. et al. (1990) Cell 63:1149. Saito, M. et al. (1992) J. Immunol. 148:4066. Novick, D. et al. (1989) J. Exp. Med. 170:1409. Jones, S.A. et al. (2001) FASEB J. 15:43. Jones, S.A. and S. Rose-John (2002) Biochim. Biophys. Acta 1592:251.
Long Name	Interleukin 6 Receptor alpha
Entrez Gene IDs	3570 (Human); 16194 (Mouse); 24499 (Rat); 102116430 (Cynomolgus Monkey)
Alternate Names	CD126, IL-6 R alpha, IL-6R alpha, IL-6R-1, IL-6Ra, IL6Q, IL6R, IL6R alpha, IL6RA, IL6RQ, gp80, interleukin 6 receptor

Citations for Mouse IL-6R alpha Antibody (3)

Showing 1 - 3 of 3 citations Showing All

Filter By:

Search Title:

Citations are publications that use Bio-Techne products. Selected citations for Mouse IL-6R alpha Antibody include:

Species: Mouse
Sample Types: Whole Cells
Applications: IHC-P

Ke F et al. (2014), Autocrine interleukin-6 drives skin-derived mesenchymal stem cell trafficking via regulating voltage-gated Ca(2+) channels. Stem Cells, 32(10):2799-810.
PMID: 24906203
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry

Bailey et al. (2012), Ifitm3 limits the severity of acute influenza in mice. PLoS Pathog, 8(9):e1002909.
PMID: 22969429
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr

Matsumoto S et al. (2010), Essential roles of IL-6 trans-signaling in colonic epithelial cells, induced by the IL-6/soluble-IL-6 receptor derived from lamina propria macrophages, on the development of colitis-associated premalignant cancer in a murine model. J. Immunol., 184(3):1543-51.
PMID: 20042582

There are no citations that match your criteria.

Showing 1 - 3 of 3 citations Showing All

Product Documents for Mouse IL-6R alpha Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS