Mouse IL-6R alpha Antibody

Name: Mouse IL-6R alpha Antibody
Brand: R&D Systems
SKU: MAB18301
Rating: 5 (3 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # MAB18301

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(3)

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Key Product Details

Species Reactivity:	Mouse
Immunogen:	Mouse myeloma cell line NS0-derived recombinant mouse IL-6R alpha Leu20-Glu357 Accession # P22272
Applications:	Immunocytochemistry/Immunofluorescence, Western Blot
Label:	Unconjugated
Source:	Monoclonal Rat IgG₁ Clone # 255820
Specificity:	Detects mouse IL-6R alpha in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human IL-6 R is observed.

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Applications for Mouse IL-6R alpha Antibody

	Recommended Concentration	Sample
Western Blot	1 µg/mL	Recombinant Mouse IL-6R alpha (Catalog # 1830-SR)
Immunocytochemistry	8-25 µg/mL	See below

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Mouse IL-6R alpha Antibody

	Immunocytochemistry IL-6R alpha in Mouse Splenocytes. IL-6R alpha was detected in immersion fixed mouse splenocytes using Rat Anti-Mouse IL-6R alpha Monoclonal Antibody (Catalog # MAB18301) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Endotoxin level	<0.10 EU per 1 μg of the antibody by the LAL method.
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: IL-6R alpha

Interleukin 6 (IL-6) is a multifunctional cytokine that exerts its activities by binding to a high-affinity receptor complex consisting of two membrane glycoproteins: an 80 kDa ligand binding subunit (IL-6 R alpha /CD126) and a 130 kDa nonligand-binding signal-transducing subunit (gp130/CD130) (1‑4). The mouse IL-6 R alpha cDNA encodes a precursor type I transmembrane protein of 460 amino acids (aa) that contains a 19 aa signal sequence, a 345 aa extracellular ligand binding domain, a 21 aa transmembrane region, and a 75 aa cytoplasmic segment (2). The extracellular segment contains an Ig-like and a fibronectin-type III domain, plus a membrane proximal WSXWS motif. In their extracellular regions, mouse IL‑6 R alpha shares 89%, 51% and 50% aa identity with rat, human and porcine IL‑6 R alpha, respectively. Unlike gp130 that is expressed ubiquitously, the cellular distribution of IL-6 R alpha is predominantly limited to hepatocytes and leukocyte subpopulations such as monocytes, neutrophils, T and B cells. Soluble IL-6R alpha has been found in various body fluids (5). Two soluble receptor isoforms that arise either from proteolytic cleavage of the membrane-bound IL‑6 R alpha, or by alternative mRNA splicing (reported only in human) have been described (6, 7). Soluble IL-6 R alpha binds IL-6 with an affinity similar to that of the membrane-bound IL-6 R alpha. More importantly, the soluble IL-6 R alpha /IL-6 complex is capable of interacting with the membrane-bound gp130 to activate cells that lack an integral membrane IL-6 R alpha. It has been documented that elevated soluble IL-6 R is associated with numerous diseases including arthritic lesions, multiple myeloma and Crohn’s disease (6, 7).

Item	Value
References	Yamasaki, K. et al. (1988) Science 241:825. Sugita, T. et al. (1990) J. Exp. Med. 171:2001. Hibi, M. et al. (1990) Cell 63:1149. Saito, M. et al. (1992) J. Immunol. 148:4066. Novick, D. et al. (1989) J. Exp. Med. 170:1409. Jones, S.A. et al. (2001) FASEB J. 15:43. Jones, S.A. and S. Rose-John (2002) Biochim. Biophys. Acta 1592:251.
Long Name	Interleukin 6 Receptor alpha
Entrez Gene IDs	3570 (Human); 16194 (Mouse); 24499 (Rat); 102116430 (Cynomolgus Monkey)
Alternate Names	CD126, IL-6 R alpha, IL-6R alpha, IL-6R-1, IL-6Ra, IL6Q, IL6R, IL6R alpha, IL6RA, IL6RQ, gp80, interleukin 6 receptor

Citations for Mouse IL-6R alpha Antibody (1)

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Citations are publications that use Bio-Techne products. Selected citations for Mouse IL-6R alpha Antibody include:

Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

Sun B et al. (2010), Sertoli cell-initiated testicular innate immune response through toll-like receptor-3 activation is negatively regulated by Tyro3, Axl, and mer receptors. Endocrinology, 151(6):2886-97.
PMID: 20363878

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Customer Reviews for Mouse IL-6R alpha Antibody (3)

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Mouse IL-6R alpha Antibody

Name: Anonymous

Application: ICC/IF

Sample Tested: Cancerous basophil cells

Species: Mouse

Verified Customer | Posted 12/14/2021
Mouse IL-6R alpha Antibody

Name: Anonymous

Application: WB

Sample Tested: Bone marrow cells

Species: Mouse

Verified Customer | Posted 05/20/2019
Mouse IL-6 R alpha Antibody

Name: Anonymous

Application: WB

Sample Tested: RAW 264.7 mouse monocyte/macrophage cell line

Species: Mouse

Verified Customer | Posted 07/15/2018

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Product Documents for Mouse IL-6R alpha Antibody

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Mouse IL-6R alpha Antibody

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Complete Your Research

Complete Your Experiment

Applications for Mouse IL-6R alpha Antibody

Scientific Data Examples for Mouse IL-6R alpha Antibody

Immunocytochemistry

Formulation, Preparation and Storage

Reconstitution Calculator

Background: IL-6R alpha

Citations for Mouse IL-6R alpha Antibody (1)

Customer Reviews for Mouse IL-6R alpha Antibody (3)

Customer Images

Mouse IL-6R alpha Antibody

Mouse IL-6R alpha Antibody

Mouse IL-6 R alpha Antibody

Product Documents for Mouse IL-6R alpha Antibody

Product Datasheets

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