Mouse IL-21R Antibody

Name: Mouse IL-21R Antibody
Brand: R&D Systems
SKU: AF596

R&D Systems, Inc. a Bio-Techne Brand

Catalog # AF596

Citations (1)

Product Datasheet / COA / SDS

Catalog #

Availability

Size / Price

Qty

Setting the standard in quality research reagents

Key Product Details

Species Reactivity:	Mouse
Immunogen:	S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse IL-21 R Leu21-Pro236 Accession # Q9JHX3
Applications:	CyTOF-ready, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Western Blot
Label:	Unconjugated
Source:	Polyclonal Goat IgG
Specificity:	Detects mouse IL-21 R in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross‑reactivity with recombinant human IL‑21 R is observed, and less than 1% cross-reactivity will recombinant mouse IL-2 R beta is observed.

View Full Technical Details for Mouse IL-21R Antibody

Applications for Mouse IL-21R Antibody

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Mouse IL‑21 R Subunit Fc Chimera (Catalog # 596-MR)
Flow Cytometry	2.5 µg/10⁶ cells	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry	5-15 µg/mL	Immersion fixed mouse splenocytes

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Mouse IL-21R Antibody

	Flow Cytometry Detection of IL‑21 R in A20 Mouse Cell Line by Flow Cytometry. A20 mouse B cell lymphoma cell line was stained with Goat Anti-Mouse IL-21 R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF596, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).
	Immunocytochemistry IL‑10 in Mouse Splenocytes. IL‑10 was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse/Rat IL‑10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF519) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells<.>

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: IL-21R

The interleukin-21 (IL-21) and its receptor appear to play important roles in the regulation of the immune system. IL-21 R, also called NILR (novel interleukin receptor) is a type I cytokine receptor with 4 conserved cysteine residues and an extracellular WSXWS motif. It is most closely related to IL-2 R beta and IL-4 R alpha. Mouse IL-21 R is a 529 amino acid (aa) residue protein with a 19 aa signal peptide, a 217 aa extracellular domain, an 18 aa transmembrane domain, and a 275 aa cytoplasmic domain. Mouse and human IL-21 R share 62% aa identity. IL-21 R is expressed on lymphoid tissues, peripheral B cells, and cell lines of T, B and natural killer lineage. Although not fully elucidated, the IL-2 R gamma ( gamma _c) chain appears to play a role in IL-21 R signaling. The IL-21/IL-21 R interaction appears to play important roles in B and T cell proliferation after antigen stimulation and natural killer cell maturation.

Item	Value
References	Parrish-Novak, J. et al. (2000) Nature 408:57. Ozaki, K. et al. (2000) Proc. Natl. Acad. Sci. USA 97:11439. Dumoutier, L. et al. (2000) Proc. Natl. Acad. Sci. USA 97:10144. Asao, H. et al. (2001) J. Immunol. 167:1.
Long Name	Interleukin 21 Receptor
Entrez Gene IDs	50615 (Human); 60504 (Mouse); 102135042 (Cynomolgus Monkey); 705759 (Rhesus Macaque)
Alternate Names	CD360, CD360 antigen, IL-21 R, IL-21 receptor, IL-21R, IL21R, MGC10967, NILR, Novel interleukin receptor, interleukin 21 receptor, interleukin-21 receptor

Citations for Mouse IL-21R Antibody (1)

Showing 1 - 1 of 1 citation Showing All

Filter By:

Search Title:

Citations are publications that use Bio-Techne products. Selected citations for Mouse IL-21R Antibody include:

Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry

X Zhang et al. (2019), IL-11 Induces Encephalitogenic Th17 Cells in Multiple Sclerosis and Experimental Autoimmune Encephalomyelitis J. Immunol., 0(0).
PMID: 31341075

There are no citations that match your criteria.

Showing 1 - 1 of 1 citation Showing All

Product Documents for Mouse IL-21R Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Mouse IL-21R Antibody

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Customers also Viewed

Customers also Purchased

Applications for Mouse IL-21R Antibody

Scientific Data Examples for Mouse IL-21R Antibody

Flow Cytometry

Immunocytochemistry