Mouse IL-10 R alpha Antibody

Name: Mouse IL-10 R alpha Antibody
Brand: R&D Systems
SKU: AF-474-NA

R&D Systems, Inc. a Bio-Techne Brand

Catalog # AF-474-NA

Citations (9)

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Species Reactivity:	Mouse
Immunogen:	S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse IL‑10 R alpha Thr24-Asn238 (Lys188Arg and Glu222Gly) Accession # Q61727
Applications:	Block/Neutralize, Western Blot
Label:	Unconjugated
Source:	Polyclonal Goat IgG
Specificity:	Detects mouse IL-10 R alpha in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 25% cross-reactivity with recombinant human IL-10 R alpha is observed.

View Full Technical Details for Mouse IL-10 R alpha Antibody

Applications for Mouse IL-10 R alpha Antibody

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Mouse IL‑10 R alpha (Catalog # 474-MR)

Item	Value
Neutralization	Measured by its ability to neutralize IL‑10-induced proliferation in the MC/9‑2 mouse mast cell line [Thompson-Snipes, L. et al. (1991) J. Exp. Med. 173:507]. The Neutralization Dose (ND₅₀) is typically 1-3 µg/mL in the presence of 1 ng/mL Recombinant Mouse IL‑10.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Mouse IL-10 R alpha Antibody

	Neutralization Cell Proliferation Induced by IL‑10 and Neutralization by Mouse IL‑10 R alpha Antibody. Recombinant Mouse IL‑10 (Catalog # 417-ML) stimulates proliferation in the MC/9‑2 mouse mast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑10 (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL‑10 Ra Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-474-NA). The ND₅₀ is typically 1-3 µg/mL.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Endotoxin level	<0.10 EU per 1 μg of the antibody by the LAL method.
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: IL-10 R alpha

IL-10, initially designated cytokine synthesis inhibitory factor (CSIF), is a potent immunosuppressant of macrophage functions. IL-10 is also a pleiotropic cytokine with multiple immunostimulatory as well as immunosuppressive effects on a variety of other cell types. IL-10 binds specifically and with high affinity to cell-surface receptors. Mouse and human cDNA clones encoding the ligand-binding IL-10 receptor (IL-10 R) have been isolated. The IL-10 R mRNA has been detected in all cell types that are known to respond to IL-10.

Human and mouse IL-10 receptors are structurally related to the IFN-gamma receptor. These receptors are members of the class II subgroup of the cytokine receptor superfamily. The deduced amino acid sequence of human IL-10 R is approximately 60% identical to mouse IL-10 R. Although human IL-10 has cross-species activities and is active on mouse cells, mouse IL-10 is species-specific in its actions and does not bind to the human IL-10 receptor. The human IL-10 R gene has been mapped to chromosome 11q23.3. Recombinant IL-10 soluble receptor, consisting of the extracellular domain of IL-10 R, binds IL-10 with high affinity in solution and is a potent IL-10 antagonist.

Item	Value
Long Name	Interleukin 10 Receptor alpha
Entrez Gene IDs	3587 (Human); 16154 (Mouse); 102127536 (Cynomolgus Monkey)
Alternate Names	CD210 antigen, CDw210a, HIL-10R, IBD28, IL-10 R alpha, IL-10 receptor subunit alpha, IL-10R subunit 1, IL-10R1, IL-10Ra, IL10R alpha, IL10RA, IL10RIL-10R subunit alpha, Interleukin-10 receptor subunit 1, interleukin 10 receptor, alpha, interleukin-10 receptor alpha chain, interleukin-10 receptor subunit alpha

Citations for Mouse IL-10 R alpha Antibody (9)

Showing 1 - 9 of 9 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Mouse IL-10 R alpha Antibody include:

Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

RR Patel et al. (2020), IL-10 normalizes aberrant amygdala GABA transmission and reverses anxiety-like behavior and dependence-induced escalation of alcohol intake Prog Neurobiol, 0(0):101952.
PMID: 33197496
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot

WJ Dai et al. (2019), Involvement of Interleukin-10 in Analgesia of Electroacupuncture on Incision Pain Evid Based Complement Alternat Med, 2019(0):8413576.
PMID: 31885668
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry

RHP Wilbers et al. (2017), Re-evaluation of IL-10 signaling reveals novel insights on the contribution of the intracellular domain of the IL-10R2 chain PLoS ONE, 12(10):e0186317.
PMID: 29016674
Species: Mouse
Sample Types: Whole Tissue
Applications: Functional Assay

CF Chang et al. (2017), Alternative activation-skewed microglia/macrophages promote hematoma resolution in experimental intracerebral hemorrhage Neurobiol. Dis., 103(0):54-69.
PMID: 28365213
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization

Y Haddad et al. (2017), The Dendritic Cell Receptor DNGR-1 Promotes the Development of Atherosclerosis in Mice Circ. Res., 0(0).
PMID: 28607102
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization

Yaoqin Hu (2016), Mesenchymal Stem Cell-Educated Macrophages Ameliorate LPS-Induced Systemic Response Mediators Inflamm, 2016(0):3735452.
PMID: 27546994
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization

Yue Y et al. (2015), IL4I1 Is a Novel Regulator of M2 Macrophage Polarization That Can Inhibit T Cell Activation via L-Tryptophan and Arginine Depletion and IL-10 Production. PLoS ONE, 10(11):e0142979.
PMID: 26599209
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization

Lim S et al. (2013), Neuronal synapse formation induced by microglia and interleukin 10. PLoS ONE, 8(11):e81218.
PMID: 24278397
Species: Rat
Sample Types: Whole Cells
Applications: ICC

Mathurin P et al. (2002), IL-10 receptor and coreceptor expression in quiescent and activated hepatic stellate cells. Am. J. Physiol. Gastrointest. Liver Physiol., 282(6):G981-90.
PMID: 12016123

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Mouse IL-10 R alpha Antibody

R&D Systems, Inc. a Bio-Techne Brand

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Applications for Mouse IL-10 R alpha Antibody

Scientific Data Examples for Mouse IL-10 R alpha Antibody

Neutralization

Formulation, Preparation and Storage

Reconstitution Calculator

Background: IL-10 R alpha

Citations for Mouse IL-10 R alpha Antibody (9)

Product Documents for Mouse IL-10 R alpha Antibody

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