Western blot shows lysates of DA3 mouse myeloma cell line. PVDF membrane was probed with 1 µg/mL of Mouse IkB-e Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4637) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for IkB-e at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Mouse IkB-epsilon Antibody

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: DA3 mouse myeloma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IkB-epsilon

NF kappaB inhibitor epsilon (IkB-epsilon) inhibits the NF kappaB complex by binding and sequestering it in the cytoplasm. Upon stimulation, IkB-epsilon is phosphorylated on serine residues marking it for degradation by the ubiquitin pathway. Following IkB-epsilon degradation, the NF kappaB complex is able to translocate to the nucleus and activate transcription.

Long Name

I-kappa-B-epsilon

Alternate Names

IkBepsilon, NFKBIE

Entrez Gene IDs

4794 (Human); 18037 (Mouse)

Gene Symbol

NFKBIE

UniProt

O54910

Additional IkB-epsilon Products

Product Documents for Mouse IkB-epsilon Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse IkB-epsilon Antibody

For research use only

Citations
Reviews

Mouse IkB-epsilon Antibody

R&D Systems, part of Bio-Techne | Catalog # AF4637

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all IkB-epsilon Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Mouse IkB-epsilon Antibody

Detection of Mouse IkB-epsilon by Western Blot.