Detects mouse IFN-gamma R1/CD119 in ELISAs. In sandwich immunoassays, no cross-reactivity or interference with recombinant mouse (rm) IFN‑ alpha/ beta R1, rmIFN-gamma R2, recombinant human (rh) IFN-gamma R1, rhIL-20 R alpha, rhIL-20 R beta, rmIL-20 R alpha, rhIL-22BP or rmIL-22BP is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG_2B

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Mouse IFN-gamma R1/CD119 Antibody

IFN‑ gamma Inhibition of EMCV-induced Cytopathy and Neutralization by Mouse IFN‑ gamma R1/CD119 Antibody.

Recombinant Mouse IFN-gamma (Catalog # 485-MI) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by Resazurin. Inhibition of EMCV activity elicited by Recombinant Mouse IFN-gamma (1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IFN-gamma R1/CD119 Monoclonal Antibody (Catalog # MAB10262). The ND₅₀ is typically 4-20 ng/mL.

Mouse IFN-gamma R1 / CD119 ELISA Standard Curve

Recombinant Mouse IFN-gamma R1/CD119 Fc Chimera (Catalog # 1026-GR) was serially diluted and captured by Rat Anti-Mouse IFN-gamma R1/CD119 Monoclonal Antibody (Catalog # MAB10262) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IFN-gamma R1/CD119 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1026) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Mouse IFN-gamma R1/CD119 Antibody

Application

Recommended Usage

Neutralization

Measured by its ability to neutralize IFN‑ gamma R1/CD119-mediated inhibition of EMCV-induced cytopathy in the L‑929 mouse fibroblast cell line. Vogel, S. N. et al. (1982) Infect. Immun. 38:681. The Neutralization Dose (ND₅₀) is typically 4-20 ng/mL in the presence of 1 ng/mL Recombinant Mouse IFN‑ gamma.

Mouse IFN-gamma R1 Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)

Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:

Detection Reagent: Mouse IFN-gamma R1/CD119 Biotinylated Antibody (Catalog # BAF1026)
Standard: Recombinant Mouse IFN-gamma R1 Fc Chimera Protein, CF (Catalog # 1026-GR)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IFN-gamma R1/CD119

The high-affinity IFN-gamma receptor complex is made up of two type I membrane proteins, IFN-gamma R1 (IFN-gamma R alpha) and IFN-gamma R2 (IFN-gamma R beta). Both proteins are members of the type II cytokine receptor family and share approximately 52% overall sequence identity. IFN-gamma R1 is the ligand-binding subunit that is necessary and sufficient for IFN-gamma binding and receptor internalization. IFN-gamma R2 is required for IFN-gamma signaling but does not bind IFN-gamma by itself. Human IFN-gamma R1 cDNA encodes a 499 amino acid (aa) protein with a 17 aa signal peptide, a 228 aa extracellular domain, a 23 aa transmembrane domain, and a 221 aa intracellular domain. Human and mouse IFN-gamma R1 share 52% amino acid sequence similarity and bind IFN-gamma in a species-specific manner. IFN-gamma R1 is constitutively expressed in most cell types. Soluble IFN-gamma R1 that binds IFN-gamma has been detected in biological fluids.

References

Bach, E.A. et al. (1997) Annu. Rev. Immunol. 15:563.

Long Name

Interferon gamma Receptor 1

Alternate Names

CD119, IFN-gR1, IFNgammaR1, IFNGR1

Entrez Gene IDs

3459 (Human); 15979 (Mouse)

Gene Symbol

IFNGR1

UniProt

NP_034641

Additional IFN-gamma R1/CD119 Products

Product Documents for Mouse IFN-gamma R1/CD119 Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Mouse IFN-gamma R1/CD119 Antibody

For research use only

Citations
Reviews

Mouse IFN-gamma R1/CD119 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB10262

Key Product Details

Species Reactivity

Validated:

Cited:

Applications