Detects mouse IFN‑ alpha/ beta R2 in direct ELISAs and Western blots. In Western blots, approximately 10% cross-reactivity with recombinant mouse (rm) IFN-gamma R2 and rmIL-20 R alpha is observed, 5% cross-reactivity with recombinant human (rh) IL-10 R beta, rmIFN-gamma RI, and rhIL-20 R beta is observed and less than 1% cross-reactivity with rhIFN-beta R alpha is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Mouse IFN-alpha / beta R2 Antibody

Limitin Inhibition of EMCV-induced Cytopathy and Neutralization by Mouse IFN‑ alpha/ beta R2 Antibody.

Recombinant Mouse Limitin (1535-LM) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line). Inhibition of EMCV activity elicited by Recombinant Mouse Limitin (30 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IFN-a/ beta R2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1083). The ND₅₀ is typically 2-10 µg/mL.

Detection of IFN-alpha / beta R2 in A20 cells by Flow Cytometry

A20 cells were stained with Goat Anti-Mouse IFN-alpha / beta R2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1083, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram) followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse IFN-alpha / beta R2 Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: A20 cells

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse IFN-alpha / beta R2

Neutralization

Measured by its ability to neutralize IFN‑ alpha/ beta R2-mediated inhibition of EMCV-induced cytopathy in the L‑929 mouse fibroblast cell line. The Neutralization Dose (ND₅₀) is typically 2-10 µg/mL in the presence of 30 pg/mL Recombinant Mouse Limitin.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IFN-alpha/beta R2

IFN-alpha / beta R2 belongs to the type II cytokine receptor family. It complexes with IFN-alpha / beta R1 to form the signaling receptor complex for the family of alpha and beta IFN subtypes. By alternative splicing, IFN-alpha / beta R2 can exist as a secreted soluble protein or as a type I membrane protein.

Long Name

Interferon alpha/beta Receptor 2

Alternate Names

IFN-aR2, IFN-bR2, IFNAR2, IFNbR2

Entrez Gene IDs

3455 (Human); 15976 (Mouse); 102140083 (Cynomolgus Monkey)

Gene Symbol

IFNAR2

UniProt

Q9D1R7

Additional IFN-alpha/beta R2 Products

Product Documents for Mouse IFN-alpha / beta R2 Antibody

Product Datasheets

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COA

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Product Specific Notices for Mouse IFN-alpha / beta R2 Antibody

For research use only

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