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Recombinant Mouse Limitin/IFN-zeta Protein

Catalog # 1535-LM | R&D Systems, Inc. a Bio-Techne Brand
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Carrier Free
1535-LM-010/CF

Catalog #
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With Carrier
1535-LM-010

Key Product Details

Source

NS0

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Limitin/IFN-zeta protein
Leu22-Arg182, with a C-terminal Ile and 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu22

Predicted Molecular Mass

19 kDa

SDS-PAGE

25 kDa, reducing conditions

Activity

Measured in an anti-viral assay using L‑929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus. Vogel, S.N. et al. (1982) Infect. Immunol. 38:681.
The ED50 for this effect is 3-15 pg/mL.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 1535-LM
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 1535-LM/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Limitin/IFN-zeta

Limitin is a secreted interferon (IFN)-like glycoprotein. It has antiviral activity and selectively inhibits B-lineage lymphoid-cell development both in vivo and in vitro, while having little effect on myelopoiesis or erythropoiesis. Mouse limitin cDNA encodes a 182 aa residue precursor protein with an N-terminal 21 aa residue putative signal peptide. Limitin binds to the IFN-alpha / beta receptors and induces IFN regulatory factor-1. Mouse limitin displays 32%, 26% and 30% amino acid identity with mouse IFN-alpha, mouse IFN-beta, and human IFN-omega, respectively (1 - 2).

References

  1. Oritani, K. et al. (2001) J. Mol. Med. 79:168.
  2. Oritani, K. et al. (2000) Nature Med. 6:659.

Long Name

Interferon zeta

Alternate Names

IFN-z, IFNzeta

Entrez Gene IDs

319146 (Mouse)

Gene Symbol

IFNZ

UniProt

Additional Limitin/IFN-zeta Products

Product Documents for Recombinant Mouse Limitin/IFN-zeta Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse Limitin/IFN-zeta Protein

For research use only

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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