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Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry

Label

Alexa Fluor 405 (Excitation = 405 nm, Emission = 421 nm)

Antibody Source

Monoclonal Rat IgG2A Clone # 744509

Product Specifications

Immunogen

E. coli-derived recombinant mouse HSF1
Thr124-Tyr247
Accession # P38532

Specificity

Detects mouse HSF1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human HSF2 or recombinant mouse HSF4 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications for Mouse HSF1 Alexa Fluor® 405-conjugated Antibody

Application
Recommended Usage

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: HSF1

Heat Shock Factor 1 (HSF1) is a 75-80 kDa constitutively expressed protein that functions as a transcriptional regulator under heat shock conditions. Heat stress induces the serine hyperphosphorylation, sumoylation, trimerization, and translocation of HSF1 to the nucleus. Nuclear HSF1 binds to heat shock elements (HSE) and promotes the transcription of genes important in cell protection from heat stress. Mouse HSF1 contains a DNA binding domain (aa 16-120), two hydrophobic oligomerization motifs (aa 137-212 and aa 378-407), a regulatory domain (aa 221-310), and a transactivation domain (aa 367-525). Alternate splicing of mouse HSF1 generates a short isoform that lacks 22 amino acids of the transactivation domain. Within aa 124-247, mouse HSF1 shares 94% and 99% aa sequence identity with human and rat HSF1, respectively.

Long Name

Heat Shock Transcription Factor 1

Alternate Names

HSTF1

Entrez Gene IDs

3297 (Human); 154991 (Mouse); 79245 (Rat)

Gene Symbol

HSF1

UniProt

Additional HSF1 Products

Product Documents for Mouse HSF1 Alexa Fluor® 405-conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse HSF1 Alexa Fluor® 405-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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