Mouse Galectin-1 Antibody

Name: Mouse Galectin-1 Antibody
Brand: R&D Systems
SKU: MAB12451

R&D Systems, Inc. a Bio-Techne Brand

Catalog # MAB12451

Citations (1)

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Species Reactivity:	Mouse
Immunogen:	E. coli-derived recombinant mouse Galectin-1 Ala2-Glu135 Accession # P16045
Applications:	Immunohistochemistry, Simple Western, Western Blot
Label:	Unconjugated
Source:	Monoclonal Rat IgG_2B Clone # 201002
Specificity:	Detects mouse Galectin-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant human (rh) Galectin-1 is observed, and no cross-reactivity with rhGalectin-2, recombinant mouse (rm) Galectin-3, rmGalectin‑4, rmGalectin‑7, or rhGalectin‑8 is observed.

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Applications for Mouse Galectin-1 Antibody

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	THP‑1 human acute monocytic leukemia cells, C2C12 mouse myoblast cells, Neuro‑2A mouse neuroblastoma cells and Mouse Thymus
Simple Western	50 µg/mL	C2C12 mouse myoblast cell line
Immunohistochemistry	8-25 µg/mL	Perfusion fixed frozen sections of mouse thymus

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Mouse Galectin-1 Antibody

	Simple Western Detection of Mouse Galectin‑1 by Simple Western^TM. Simple Western lane view shows lysates of C2C12 mouse myoblast cell line, loaded at 0.2 mg/mL. A specific band was detected for Galectin‑1 at approximately 18 kDa (as indicated) using 50 µg/mL of Rat Anti-Mouse Galectin‑1 Monoclonal Antibody (Catalog # MAB12451) followed by 1:50 dilution of HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.
	Western Blot Detection of Mouse Galectin‑1 by Western Blot. Western blot shows lysates of THP‑1 human acute monocytic leukemia cells, C2C12 mouse myoblast cells, Neuro‑2A mouse neuroblastoma cells and Mouse Thymus. PVDF membrane was probed with 0.1 µg/mL of Rat Anti-Mouse Galectin‑1 Monoclonal Antibody (Catalog # MAB12451) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Galectin‑1 at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: Galectin-1

The galectins constitute a large family of carbohydrate-binding proteins with specificity for N-acetyl-lactosamine-containing glycoproteins. At least 14 mammalian galectins, which share structural similarities in their carbohydrate recognition domains (CRD), have been identified to date. The galectins have been classified into the prototype galectins (-1, -2, -5, -7, -10, -11, -13, -14), which contain one CRD and exist either as a monomer or a noncovalent homodimer; the chimera galectins (Galectin-3) containing one CRD linked to a nonlectin domain; and the tandem-repeat galectins (-4, -6, -8, -9, -12) consisting of two CRDs joined by a linker peptide. Galectins lack a classical signal peptide and can be localized to the cytosolic compartments where they have intracellular functions. However, via one or more as yet unidentified non-classical secretory pathways, galectins can also be secreted to function extracellularly. Individual members of the galectin family have different tissue distribution profiles and exhibit subtle differences in their carbohydrate-binding specificities. Each family member may preferentially bind to a unique subset of cell-surface glycoproteins (1-4). Mouse Galectin-1, also known as beta-galactoside-binding lectin L-14-I, lactose-binding lectin 1, S-Lac lectin 1, galaptin and 14 kDa lectin, is a monomeric or homodimeric prototype galectin that is expressed in a variety of cells and tissues including muscle, heart, lymph nodes, spleen, thymus, macrophages, B cells, T cells, dendritic cells, and tumor cells. It preferentially binds laminin, fibronectin, 90K/Mac-2BP, CD45, CD43, CD7, CD2, CD3, and ganglioside GM1. Galectin-1 modulates cell growth, proliferation and differentiation, either positively or negatively, depending on the cell type and activation status. It controls cell survival by inducing apoptosis of activated T cells and immature thymocytes. It modulates cytokine secretion by inducing Th2 type cytokines and inhibiting pro-inflammatory cytokine production. Galectin-1 can also modulate cell-cell as well as cell-matrix interactions and depending on the cell type and developmental stage, promote cell attachment or detachment. Galectin-1 has immunosuppressive and anti-inflammatory properties and has been shown to suppress acute and chronic inflammation and autoimmunity. Mouse and human Galectin-1 share about 88% amino acid sequence similarity (1-5).

Item	Value
References	Rabinovich, A. et al. (2002) Trends in Immunol. 23:313. Rabinovich, A. et al. (2002) J. Leukocyte Biology 71:741. Hughes, R.C. (2001) Biochimie 83:667. R&D Systems’ Cytokine Bulletin; Summer 2002. Goldring, K. et al. (2002) J. Cell Science 115:355.
Entrez Gene IDs	3956 (Human); 16852 (Mouse)
Alternate Names	14 kDa laminin-binding protein, 14 kDa lectin, BHL, Beta-galactoside-binding lectin L-14-I, DKFZp686E23103, GAL1, GBP, Galaptin, Galectin-1, Galectin1, HBL, HLBP14, HPL, L-14, LGALS1, Lactose-binding lectin 1, Lectin galactoside-binding soluble 1, Putative MAPK-activating protein PM12, S-Lac lectin 1, gal-1, galectin 1, lectin, galactoside-binding, soluble, 1

Citations for Mouse Galectin-1 Antibody (1)

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Citations are publications that use Bio-Techne products. Selected citations for Mouse Galectin-1 Antibody include:

Species: Human
Sample Types: Whole Tissue
Applications: IHC-P

Wiest I et al. (2012), Expression of different carbohydrate tumour markers and galectins 1 and 3 in normal squamous and malignant epithelia of the upper aaerodigestive tract. Anticancer Res., 32(5):2023-9.
PMID: 22593483

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Mouse Galectin-1 Antibody

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Scientific Data Examples for Mouse Galectin-1 Antibody

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Formulation, Preparation and Storage

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Background: Galectin-1

Citations for Mouse Galectin-1 Antibody (1)

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