Mouse G-CSF Antibody

Name: Mouse G-CSF Antibody
Brand: R&D Systems
SKU: MAB414
Rating: 4 (3 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # MAB414

Citations (35)

(3)

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Key Product Details

Species Reactivity:	Mouse
Immunogen:	E. coli-derived recombinant mouse G-CSF Val31-Ala208 Accession # P09920
Applications:	Block/Neutralize, ELISA Capture, Flow Cytometry, Western Blot
Label:	Unconjugated
Source:	Monoclonal Rat IgG₁ Clone # 67604
Specificity:	Detects mouse G-CSF in ELISAs and Western blots. In ELISAs, does not cross-react with recombinant human (rh) G‑CSF, rhCNTF, rmIL-6, rmLIF, or rmOSM.

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Applications for Mouse G-CSF Antibody

	Recommended Concentration	Sample
Western Blot	1 µg/mL	Recombinant Mouse G-CSF (Catalog # 414-CS)
Intracellular Staining by Flow Cytometry	0.25 µg/10⁶ cells	See below

Mouse G-CSF Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

2-8 µg/mL

Use in combination with:

Detection Reagent: Mouse G‑CSF Biotinylated Antibody (Catalog # BAF414)

Standard: Recombinant Mouse G-CSF Protein (Catalog # 414-CS)

Item	Value
Neutralization	Measured by its ability to neutralize G‑CSF-induced proliferation in the NFS‑60 mouse myeloid cells line. Shirafuji, N. et al. (1989) Exp. Hematol. 17:116. The Neutralization Dose (ND₅₀) is typically 0.01-0.03 µg/mL in the presence of 0.125 ng/mL Recombinant Mouse G‑CSF.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Mouse G-CSF Antibody

	Intracellular Staining by Flow Cytometry Detection of G‑CSF in Mouse splenocytes by Flow Cytometry. Mouse splenocytes treated with LPS (100 µg/ml) for 24 hours were stained with Rat Anti-Mouse G-CSF Monoclonal Antibody (Catalog # MAB414, filled histogram) or isotype control antibody (Catalog # MAB005, open histogram) followed by Anti-Rat IgG PE-conjugated secondary antibody (Catalog # F0105B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
	Neutralization Cell Proliferation Induced by G‑CSF and Neutralization by Mouse G‑CSF Antibody. Recombinant Mouse G-CSF (Catalog # 414-CS) stimulates proliferation in the NFS-60 mouse myeloid cells line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse G-CSF (0.125 ng/mL) is neutralized (green line) by increasing concentrations of Mouse G-CSF Monoclonal Antibody (Catalog # MAB414). The ND₅₀ is typically 0.01-0.03 µg/mL.

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Endotoxin level	<0.10 EU per 1 μg of the antibody by the LAL method.
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: G-CSF

G-CSF is a pleiotropic cytokine best known for its specific effects on the proliferation, differentiation, and activation of hematopoietic cells of the neutrophilic granulocyte lineage. It is produced mainly by monocytes and macrophages upon activation by endotoxin, TNF-alpha and IFN-gamma. Other cell types including fibroblasts, endothelial cells, astrocytes and bone marrow stromal cells can also secrete G-CSF after LPS, IL-1, or TNF-alpha activation. In addition, various carcinoma cell lines and myeloblastic leukemia cells can express G-CSF constitutively.

The murine G-CSF cDNA encodes a 208 amino acid (aa) residue precursor protein containing a 30 aa residue signal peptide that is proteolytically cleaved to generate the 178 aa residue mature protein. Human G-CSF is 73% identical at the amino acid level to murine G-CSF and the two proteins show species cross-reactivity.

In vitro, G-CSF stimulates growth, differentiation and functions of cells from the neutrophil lineage. It also has blast cell growth factor activity and can synergize with IL-3 to shorten the G_o period of early hematopoietic progenitors. Consistent with its in vitro functions, G-CSF has been found to play important roles in defense against infection, in inflammation and repair, and in the maintenance of steady state hematopoiesis. Recombinant human G-CSF has been approved for the amelioration of chemotherapy induced neutropenia as well as for severe chronic neutropenia following marrow transplant.

Item	Value
Long Name	Granulocyte Colony Stimulating Factor
Entrez Gene IDs	1440 (Human); 12985 (Mouse)
Alternate Names	C17orf33, CSF3, CSF3OS, Filgrastim, G-CSF, GCSF, GCSFlenograstim, Lenograstim, MGC45931, Pluripoietin, chromosome 17 open reading frame 33, colony stimulating factor 3 (granulocyte), granulocyte colony-stimulating factor

Citations for Mouse G-CSF Antibody (35)

Showing 1 - 10 of 35 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Mouse G-CSF Antibody include:

Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

DW Kwak et al. (2022), Leukotriene B4 Receptor 2 Mediates the Production of G-CSF That Plays a Critical Role in Steroid-Resistant Neutrophilic Airway Inflammation Biomedicines, 10(11).
PMID: 36428547
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

H Yu et al. (2021), Granulocyte colony-stimulating factor (G-CSF) mediates bone resorption in periodontitis BMC Oral Health, 21(1):299.
PMID: 34118920
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry

CS Umeshappa et al. (2021), Liver-specific T regulatory type-1 cells program local neutrophils to suppress hepatic autoimmunity via CRAMP Cell Reports, 34(13):108919.
PMID: 33789099
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization

M Goedhart et al. (2021), Bone Marrow Harbors a Unique Population of Dendritic Cells with the Potential to Boost Neutrophil Formation upon Exposure to Fungal Antigen Cells, 11(1).
PMID: 35011617
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

S Ouyang et al. (2020), Targeting IL-17A/glucocorticoid synergy to CSF3 expression in neutrophilic airway diseases JCI Insight, 5(3).
PMID: 32051346
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

F Sparber et al. (2018), Langerin+ DCs regulate innate IL-17 production in the oral mucosa during Candida albicans-mediated infection PLoS Pathog., 14(5):e1007069.
PMID: 29782555
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization

MA Meyer et al. (2018), Breast and pancreatic cancer interrupt IRF8-dependent dendritic cell development to overcome immune surveillance Nat Commun, 9(1):1250.
PMID: 29593283
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization

JM Ubellacker et al. (2018), Modulating bone marrow hematopoietic lineage potential to prevent bone metastasis in breast cancer Cancer Res., 0(0).
PMID: 30065048
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization

J Banuelos et al. (2017), Granulocyte colony-stimulating factor blockade enables dexamethasone to inhibit lipopolysaccharide-induced murine lung neutrophils PLoS ONE, 12(5):e0177884.
PMID: 28542361
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization

H Zhu et al. (2017), Reactive Oxygen Species-Producing Myeloid Cells Act as a Bone Marrow Niche for Sterile Inflammation-Induced Reactive Granulopoiesis J. Immunol, 0(0).
PMID: 28235862
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

Bohannon J et al. (2016), Role of G-CSF in monophosphoryl lipid A-mediated augmentation of neutrophil functions after burn injury. J Leukoc Biol, 99(4):629-40.
PMID: 26538529
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization

Sivakumaran S et al. (2016), Depletion of CD11c(+) cells in the CD11c.DTR model drives expansion of unique CD64(+) Ly6C(+) monocytes that are poised to release TNF-alpha. Eur J Immunol, 46(1):192-203.
PMID: 26464217
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization

Anna A Müller (2016), An NK Cell Perforin Response Elicited via IL-18 Controls Mucosal Inflammation Kinetics during Salmonella Gut Infection PLoS Pathog, 12(6):e1005723.
PMID: 27341123
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization

Arna E Andrews (2016), Therapeutic Targeting of the G-CSF Receptor Reduces Neutrophil Trafficking and Joint Inflammation in Antibody-Mediated Inflammatory Arthritis J. Immunol., 0(0).
PMID: 27807194
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization

(2016), The Deubiquitinase OTULIN Is an Essential Negative Regulator of Inflammation and Autoimmunity Cell, 0(0).
PMID: 27523608
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization

(2016), Tumor-secreted products repress B-cell lymphopoiesis in a murine model of breast cancer Eur J Immunol, 0(0).
PMID: 27618761
Species: Mouse
Sample Types: In Vivo

Trautwein-Weidner K et al. (2015), IL-17-mediated antifungal defense in the oral mucosa is independent of neutrophils. Mucosal Immunol, 8(2):221-31.
PMID: 25005360
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization

Brooks R et al. (2015), Coordinate expansion of murine hematopoietic and mesenchymal stem cell compartments by SHIPi. Stem Cells, 33(3):848-58.
PMID: 25402778
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization

Coffelt S et al. (2015), IL-17-producing gammadelta T cells and neutrophils conspire to promote breast cancer metastasis. Nature, 522(7556):345-8.
PMID: 25822788
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry

Agarwal S et al. (2015), G-CSF Promotes Neuroblastoma Tumorigenicity and Metastasis via STAT3-Dependent Cancer Stem Cell Activation. Cancer Res, 75(12):2566-79.
PMID: 25908586
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization

Cedervall J et al. (2015), Neutrophil Extracellular Traps Accumulate in Peripheral Blood Vessels and Compromise Organ Function in Tumor-Bearing Animals. Cancer Res, 75(13):2653-62.
PMID: 26071254
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization

Taniguchi K et al. (2014), Periostin controls keratinocyte proliferation and differentiation by interacting with the paracrine IL-1alpha/IL-6 loop. J Invest Dermatol, 134(5):1295-304.
PMID: 24352037
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization

Gardner J et al. (2014), G-CSF drives a posttraumatic immune program that protects the host from infection. J Immunol, 192(5):2405-17.
PMID: 24470495
Species: Mouse
Sample Types: Plasma
Applications: Luminex Development

Zhang M et al. (2012), Response patterns of cytokines/chemokines in two murine strains after irradiation. Cytokine, 58(2):169-77.
PMID: 22277799
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization

Waight JD et al. (2011), Tumor-derived G-CSF facilitates neoplastic growth through a granulocytic myeloid-derived suppressor cell-dependent mechanism. PLoS ONE, 6(11):e27690.
PMID: 22110722
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization

Cain DW et al. (2011), Inflammation Triggers Emergency Granulopoiesis through a Density-Dependent Feedback Mechanism. PLoS ONE, 6(5):e19957.
PMID: 21655273
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization

Cheung R et al. (2011), Activation of MDL-1 (CLEC5A) on immature myeloid cells triggers lethal shock in mice. J. Clin. Invest., 121(11):4446-61.
PMID: 22005300
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization

Hegde VL et al. (2010), Cannabinoid receptor activation leads to massive mobilization of myeloid-derived suppressor cells with potent immunosuppressive properties. Eur. J. Immunol., 40(12):3358-71.
PMID: 21110319
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization

Shojaei F et al. (2009), G-CSF-initiated myeloid cell mobilization and angiogenesis mediate tumor refractoriness to anti-VEGF therapy in mouse models. Proc. Natl. Acad. Sci. U.S.A., 106(16):6742-7.
PMID: 19346489
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization

Furze RC et al. (2008), The role of the bone marrow in neutrophil clearance under homeostatic conditions in the mouse. FASEB J., 22(9):3111-9.
PMID: 18509199
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization

Brunner S et al. (2008), Parathyroid hormone effectively induces mobilization of progenitor cells without depletion of bone marrow. Exp. Hematol., 36(9):1157-66.
PMID: 18504066
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization

Wengner AM et al. (2007), The coordinated action of G-CSF and ELR + CXC chemokines in neutrophil mobilization during acute inflammation. Blood, 111(1):42-9.
PMID: 17928531
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization

Shojaei F et al. (2007), Bv8 regulates myeloid-cell-dependent tumour angiogenesis. Nature, 450(7171):825-31.
PMID: 18064003
Species: Mouse
Sample Types: BALF
Applications: ELISA Development

Ye P et al. (2001), Requirement of interleukin 17 receptor signaling for lung CXC chemokine and granulocyte colony-stimulating factor expression, neutrophil recruitment, and host defense. J. Exp. Med., 194(4):519-27.
PMID: 11514607
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization

Kulkarni et al. (213), Granulocyte colony-stimulating factor antibody abrogates radioprotective efficacy of gamma-tocotrienol, a promising radiation countermeasure. Cytokine,62(2):278-85.
PMID: 23561424

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Customer Reviews for Mouse G-CSF Antibody (3)

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Name: Katie Myers

Application: ELISA

Sample Tested: Serum

Species: Mouse

Verified Customer | Posted 11/14/2022

This material was use successfully as a capture antibody in our Mouse ELISA. The results were very sensitive.
Mouse G-CSF Antibody

Name: Laboratorio Inmuno-Oncología

Application: ICC/IF

Sample Tested: Melanoma tissue

Species: Human

Verified Customer | Posted 11/23/2020

Bio-Techne Response

Thank you for reviewing our product. We are sorry to hear that this antibody did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.
Mouse G-CSF Antibody

Name: Leslie Priddy

Application: IHC

Sample Tested: Serum

Species: Mouse

Verified Customer | Posted 04/13/2018

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Product Documents for Mouse G-CSF Antibody

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Mouse G-CSF Antibody

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Complete Your Research

Complete Your Experiment

Applications for Mouse G-CSF Antibody

Mouse G-CSF Sandwich Immunoassay

Scientific Data Examples for Mouse G-CSF Antibody

Intracellular Staining by Flow Cytometry

Neutralization

Formulation, Preparation and Storage

Reconstitution Calculator

Background: G-CSF

Citations for Mouse G-CSF Antibody (35)

Customer Reviews for Mouse G-CSF Antibody (3)

Customer Images

Mouse G-CSF Antibody

Mouse G-CSF Antibody

Product Documents for Mouse G-CSF Antibody

Product Datasheets

COA

Certificate of Analysis

SDS

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