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Mouse FGF‑15 Antibody

Catalog # AF6755 | R&D Systems, Inc. a Bio-Techne Brand
Catalog #
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Key Product Details

Species Reactivity






Antibody Source

Polyclonal Sheep IgG

Product Summary for Mouse FGF‑15 Antibody


E. coli-derived recombinant mouse FGF-15
Accession # O35622


Detects mouse FGF-15 in direct ELISAs. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) FGF-19 is observed and less than 5% cross-reactivity with rhFGF-22 and rhFGF-23 is observed.







Scientific Data Images for Mouse FGF‑15 Antibody

FGF-15 antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr).

FGF-15 in Mouse Embryo.

FGF-15 was detected in immersion fixed frozen sections of mouse embryo (E15) using Sheep Anti-Mouse FGF-15 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6755) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to the processes of dorsal root ganglia neurons. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Applications for Mouse FGF‑15 Antibody

Recommended Usage


5-15 µg/mL
Sample: Immersion fixed frozen sections of mouse embryo (E15)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation and Storage


Antigen Affinity-purified


Sterile PBS to a final concentration of 0.2 mg/mL.

Reconstitution Buffer Available:
Size / Price


Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FGF-15

FGF-15 (Fibroblast growth factor 15) is a 25 kDa, secreted, nonglycosylated polypeptide that is a member of the FGF-15/21/23 subfamily, FGF family of growth factors. It is synthesized by ileal epithelium, colonic myofibroblasts, and select areas of the developing brain. FGF-15 acts on hepatocytes and reduces CYP7A1 levels, thus blocking bile acid production. It also promotes colonic epithelial migration, and in the embryonic brain, inhibits over-production of neuronal precursors. FGF-15 is synthesized as a 218 amino acid (aa) precursor that contains a 25 aa signal sequence and a 182 aa mature region (aa 26-218; 23 kDa predicted MW). Mature FGF-15 contains a characteristic 3-dimensional beta-trefoil structure, plus a unique Cys pattern that contributes to stability. There are two potential splice forms. One shows a 23 aa substitution for aa 120-218, while a second shows a 41 aa substitution for aa 120-218. Over aa 23-200, mouse FGF-15 shares 96% aa identity with rat FGF-15. Although mouse FGF-15 is considered the ortholog to human FGF-19, the two molecules share only 54% aa identity over aa 23-200, and are produced by different cell types (intestinal epithelium in mouse, hepatocytes in human).

Long Name

Fibroblast Growth Factor 15

Alternate Names

FGF-19, FGF15

Entrez Gene IDs

14170 (Mouse)

Gene Symbol



Product Documents for Mouse FGF‑15 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse FGF‑15 Antibody

For research use only

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