Mouse FGF‑15 Antibody
Catalog # AF6755 | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Species Reactivity
Applications
Label
Antibody Source
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Product Summary for Mouse FGF‑15 Antibody
Immunogen
Ser24-Lys218
Accession # O35622
Specificity
Clonality
Host
Isotype
Scientific Data Images for Mouse FGF‑15 Antibody
FGF-15 in Mouse Embryo.
FGF-15 was detected in immersion fixed frozen sections of mouse embryo (E15) using Sheep Anti-Mouse FGF-15 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6755) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to the processes of dorsal root ganglia neurons. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.Applications for Mouse FGF‑15 Antibody
Immunohistochemistry
Sample: Immersion fixed frozen sections of mouse embryo (E15)
Formulation, Preparation and Storage
Purification
Reconstitution
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FGF-15
FGF-15 (Fibroblast growth factor 15) is a 25 kDa, secreted, nonglycosylated polypeptide that is a member of the FGF-15/21/23 subfamily, FGF family of growth factors. It is synthesized by ileal epithelium, colonic myofibroblasts, and select areas of the developing brain. FGF-15 acts on hepatocytes and reduces CYP7A1 levels, thus blocking bile acid production. It also promotes colonic epithelial migration, and in the embryonic brain, inhibits over-production of neuronal precursors. FGF-15 is synthesized as a 218 amino acid (aa) precursor that contains a 25 aa signal sequence and a 182 aa mature region (aa 26-218; 23 kDa predicted MW). Mature FGF-15 contains a characteristic 3-dimensional beta-trefoil structure, plus a unique Cys pattern that contributes to stability. There are two potential splice forms. One shows a 23 aa substitution for aa 120-218, while a second shows a 41 aa substitution for aa 120-218. Over aa 23-200, mouse FGF-15 shares 96% aa identity with rat FGF-15. Although mouse FGF-15 is considered the ortholog to human FGF-19, the two molecules share only 54% aa identity over aa 23-200, and are produced by different cell types (intestinal epithelium in mouse, hepatocytes in human).
Product Documents for Mouse FGF‑15 Antibody
Product Specific Notices for Mouse FGF‑15 Antibody
For research use only
Citations for Mouse FGF‑15 Antibody (1)
Citations are publications that use Bio-Techne products. Selected citations for Mouse FGF‑15 Antibody include:
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Species: Mouse
Sample Types: Serum
Applications: ELISA Development (Capture)(2016-12-24), Chronic Over-expression of Fibroblast Growth Factor 21 Increases Bile Acid Biosynthesis by Opposing FGF15/19 Action EBioMedicine, 2016-10(0).
PMID: 28041926
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Reconstitution Calculator
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